Optical detection of stress parametersKrstić, Marijana (Avtor) Bren, Urban (Mentor) Lobnik, Aleksandra (Komentor) Sliesarenko, Valeriia V. (Komentor) dopaminefluorescenceo-phthaldehydeoptical methodIn this thesis we developed the optical method of dopamine detection at pH 9, by creating DA-OPA complex. O–phthaldehyde was used as the indicator for fluorescent detection of dopamine with (3-Mercaptopropyl)-triethoxysilane as an additional reagent. The fluorescent product reached its maximum after 2 minutes, wavelengths of emission and excitation were λex/λem=340 nm/460 nm. The determination of dopamine was linear within a concentration range between 0.5∙10–6 and 3∙10–6 M. Limit of detection was 3.38∙10–6 M. Urea and norepinephrine can interfere dopamine detection, under examined parameters: ratio DA:OPA was 1:100, OPA:MPTES was 1:1, buffer was at pH 9.20232023-08-30 12:35:59Diplomsko delo/naloga85461sl