Covalent modification of chitosan surfaces with a sugar amino acid and lysine analoguesDorn, Tobias (Avtor) Finšgar, Matjaž (Avtor) Stana-Kleinschek, Karin (Avtor) Steindorfer, Tobias Alexander (Avtor) Thonhofer, Martin Simon (Avtor) Wrodnigg, Tanja (Avtor) Kargl, Rupert (Avtor) carbohydratesfluorescence spectroscopyamino acidchitosanthin flm modifcationC-GlycosidesThis work explores the modifcation and characterization of chitosan thin flms as a model for functionalized polysaccharide interfaces. The solid–liquid interface of oligo- and polysaccharides is crucial for various biological processes such as cell adhesion and recognition. By covalent surface modifcation of the chitosan via amide formation with diferent small molecules containing carboxylic acids, e.g. specially designed glycoside hydrolase inhibitors, interactions with biomolecules and living cells could potentially be controlled in the future. As a frst step towards this aim, three fuorescent compounds were conjugated onto nanometric chitosan thin flms. The layers were analysed by fuorescence spectroscopy, X-ray photoelectron spectroscopy, time-of-fight secondary ion mass spectrometry, and atomic force microscopy, to proof the covalent attachment of the target molecules. By this analysis, a uniform and chemically stable covalent attachment of the target molecules on the chitosan thin flms could be demonstrated under various conditions. This publication serves as a proof-of-concept-study for further biofunctionalization, pattering, and interaction studies involving polysaccharide interfaces, glycosidase inhibitors, proteins, or living cells.Springer20242024-08-07 08:53:47Članek v reviji89713sl© The Author(s) 2024