Optimisation of the green process of industrial hemp - preparation and its extract characterisationŽitek Makoter, Taja (Avtor) Kotnik, Petra (Avtor) Makoter, Teo (Avtor) Postružnik, Vesna (Avtor) Knez, Željko (Avtor) Knez Marevci, Maša (Avtor) supercritical fluid extractionultrasonic extractionmelanoma cellsextractionWM-266-4Natural medicines and products are becoming increasingly important in the pharmaceutical and food industries. The most important step in obtaining a natural remedy is the processing of the natural material. This study offers the separation of the industrial hemp plant into fractions by mechanical treatment, which has a significant impact on the selectivity of the obtained fractions. This study also offers a solution to reduce waste by fractionating industrial hemp, focusing on the fraction with the highest cannabinoid content (49.5% of CBD). The study confirmed the anticancer potential of the extract, which prevents further division of WM-266-4 melanoma cells at a concentration of 10−3 mg/mL. However, application of the extract (c = 10−3 mg/mL) to normal human epidermal melanocytes proved to be insignificant, as the metabolic activity of the cells was the same as in the control cell group.MDPI20222025-05-09 14:06:16Članek v reviji92747sl© 2022 by the authors