Natural Deep Eutectic Solvent-Based Matrix Solid Phase dispersion (MSPD) extraction for determination of bioactive compounds from sandy everlasting (Helichrysum arenarium L.)Ivanović, Milena (Avtor) Krajnc, Peter (Avtor) Mlinarič, Aleš (Avtor) Islamčević Razboršek, Maša (Avtor) matrix solid phase dispersion extractionMSPDnatural deep eutectic solventsNADESextraction optimizationsandy everlastingphenolic compoundsstability studiesantioxidant activityIn the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction was used to isolate the major bioactive compounds from H. arenarium. To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time, and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction with 80% MeOH was used as the standard method for the comparison of results. The stability of the obtained extracts was studied over a period of 0 to 60 days at three different temperatures (−18 ◦C, 4 ◦C, and 25 ◦C). All extracts were evaluated both spectrophotometrically (determination of total phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically (HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27% higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers, and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the studied compounds: chlorogenic acid, naringenin-40 -O-glucoside, tomoroside A, naringenin-5-Oglucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of H. arenarium polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution volume of ChCl-LA of 10 mL.MDPI20222025-05-09 14:21:10Članek v reviji92750sl© 2022 by the authors