Effect of Selected Probiotic Strains and their Combinations on the SGLT1 and GLUT2 Gene Expressions in Porcine Intestinal Epithelial CellsRakun, Lucija (Avtor) Kozmos, Maša (Mentor) Hribernik, Tamara (Komentor) Probioticsglucose transportersgene expressionCLAB cellsIntestinal morphology and glucose transporter expression are key determinants of glucose absorption and homeostasis. While probiotics beneficially modulate the gut microbiota, their role in regulating glucose transporter gene expression remains unclear. This study aimed to evaluate the effects of selected single- and multi-strain probiotic bacteria on the expression of sodium-driven glucose cotransporter 1 (SGLT1) and facilitative glucose transporter 2 (GLUT2) in porcine intestinal epithelial CLAB cells. Cells were treated with four selected probiotic strains (Lactobacillus acidophilus (ATCC 4356), Lacticaseibacillus rhamnosus GG (ATCC 53103), Lactiplantibacillus plantarum PCS 20, and Lactiplantibacillus plantarum PCS 26) at varying concentrations and in different combinations. Although none of the observed changes were statistically significant, SGLT1 expression was consistently upregulated across various treatments, whereas GLUT2 showed a more variable but predominantly downregulated response, suggesting the latter as a more susceptible target for this research. These findings indicate that selected probiotic strains may differentially regulate key intestinal glucose transporters, suggesting a potential role in modulating glucose absorption and homeostasis. Ultimately, this study offers new insights into probiotic–host interactions.  L. Rakun20262026-06-15 19:47:35Magistrsko delo/naloga98470sl