Enzymatic fatty ester synthesisPečnik, Simona (Avtor) Knez, Željko (Avtor) chemical engineeringbiotechnologyesterificationsynthesesestersenzymeslipaseMucor mieheireaction kineticsreaction thermodynamics12-isopropylidene-3-oleoyl glycerolFatty ester synthesis with immobilized 1,3-specific lipase from Mucor Miehei is described. 1,2-Isopropylidene glycerol was peoduced by condensation of glycerol with acetone was esterified with oleic acid in the presence of a Mucor Miehei lipaze (Lipozyme TM) to obtain 1,2 isopropylidene- 3-oleoyl glycerol. The effects of various process parameters (temperature and pressure)and various ratios (enzyme/substrate) have been investigated to determine optimal conditions for the esterification process. The highest conversion of oleic acid (80% w/w) was obtained at 55 oC and 57.057 bar, while the optimal addition of lipase to substrate was determined to be 0,096 g per gram of reaction mixture. The esterification can be modelled successfully as a reverse second order reaction. Thermodynamic properties of the reaction system at 55 oC and 0.057 bar were also determined. Activation energy was 20.82 kJ/mole, entropy of activation -0,26 kJ/(Kmole) and free energy of activation was 103.32 kJ/mole.19922012-06-06 14:30:27Neznano35103UDK: 66.095.13:577.15COBISS_ID: 4025092ISSN pri članku: 0003-021XNUK URN: URN:SI:UM:DK:KIDREIVQsl