Protein adsorption on various plasma-treated polyethylene-terephthalate substratesRecek, Nina (Avtor) Jaganjac, Morana (Avtor) Kolar, Metod (Avtor) Milković, Lidija (Avtor) Mozetič, Miran (Avtor) Stana-Kleinschek, Karin (Avtor) Vesel, Alenka (Avtor) oxygen and fluorine plasma treatmentpolymer surface modificationprotein adsorptioncell adhesionquartz crystal microbalanceQCMProtein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy (SEM). The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates.20132017-06-22 08:50:17Znanstveno delo66355ISSN: 1420-3049UDK: 533OceCobissID: 18462981COBISS_ID: 27136551DOI: 10.3390/molecules181012441ISSN pri članku: 1420-3049NUK URN: URN:SI:UM:DK:QQ5MC8JCsl