Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) improves immunological profile of the non-carcinogenic porcine-derived enterocytesKaraffová, Viera (Avtor) Teleky, Jana (Avtor) Kozmos, Maša (Avtor) Langerholc, Tomaž (Avtor) Mudroňová, Dagmar (Avtor) Hudec, Erik (Avtor) Ševčíková, Zuzana (Avtor) Lactobacillus reuteri B1/1CLAB cellspro-inflammatory cytokinemetabolic activityIn our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 x 10(7) and 1 x 10(9) CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1 beta, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 x 10(5) cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109 L. reuteri B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation.MDPI20232024-02-20 08:09:38Znanstveno delo87111UDK: 579COBISS_ID: 150715651DOI: 10.3390/life13051090ISSN pri članku: 2075-1729sl