Dimethoate detection through a fluorescent coumarin dyeDonà, Edoardo (Avtor) Mohr, Gerhard J. (Avtor) Lobnik, Aleksandra (Avtor) organophosphate pesticidemethylaminecoumarinfluorescence detectiondimethoateTICTtwisted intramolecular charge transferIn this study, we present a straightforward and innovative approach utilizing a coumarin fluorescent dye for the detection of dimethoate in green tea. Initially, the pesticide undergoes hydrolysis in a NaOH solution, yielding our target analyte, methylamine. Following neutralization to pH 9, methylamine reacts with the dye in CH3CN for 20 min. After a careful optimization, we achieved an outstanding linear correlation (R2 = 0.999) for dimethoate, spanning concentrations from 7.8 to 292 µg/L and LOD of 3.2 µg/L. Moreover, we successfully detected dimethoate in green tea, with a recovery of 95.4% (σ = 5.7%). Organophosphates pesticides (OPs), which dimethoate is one of the most used, pose a significant threat due to their toxicity upon both high direct exposure and prolonged low-level exposure, which has been linked to cancer. Therefore, the development of a detection method that is both selective and sensitive is imperative for safeguarding both the population and the environment. This method effectively addresses the stability challenges encountered by enzyme-based fluorescent sensors, thereby opening new avenues for the detection of organophosphate pesticides.Elsevier20242024-12-20 10:58:35Članek v reviji91424UDK: 543.426COBISS_ID: 220023811DOI: 10.1016/j.microc.2024.112205ISSN pri članku: 1095-9149sl