An overview of the current state of cell viability assessment methods using OECD classificationMadorran, Eneko (Avtor) Ambrož, Miha (Avtor) Knez, Jure (Avtor) Sobočan, Monika (Avtor) cell viabilitycell-based methodsin vitro toxicologyOECD cell viability classificationOver the past century, numerous methods for assessing cell viability have been developed, and there are many different ways to categorize these methods accordingly. We have chosen to use the Organisation for Economic Co-operation and Development (OECD) classification due to its regulatory importance. The OECD categorizes these methods into four groups: non-invasive cell structure damage, invasive cell structure damage, cell growth, and cellular metabolism. Despite the variety of cell viability methods available, they can all be categorized within these four groups, except for two novel methods based on the cell membrane potential, which we added to the list. Each method operates on different principles and has its own advantages and disadvantages, making it essential for researchers to choose the method that best fits their experimental design. This review aims to assist researchers in making this decision by describing these methods regarding their potential use and providing direct references to the cell viability assessment methods. Additionally, we use the OECD classification to facilitate potential regulatory use and to highlight the need for adding a new category to their list.MDPI20252025-02-13 12:13:55Znanstveno delo91846UDK: 576COBISS_ID: 225295363DOI: 10.3390/ijms26010220ISSN pri članku: 1422-0067sl