MFUM-BrTNBC-1, a newly established patient-derived triple-negative breast cancer cell lineSkok, Kristijan (Avtor) Gradišnik, Lidija (Avtor) Čelešnik, Helena Sabina (Avtor) Milojević, Marko (Avtor) Potočnik, Uroš (Avtor) Jezernik, Gregor (Avtor) Gorenjak, Mario (Avtor) Sobočan, Monika (Avtor) Takač, Iztok (Avtor) Kavalar, Rajko (Avtor) Maver, Uroš (Avtor) hormonal receptorsMFUM-BrTNBC-1MCF-7MDA-MB-231MDA-MB-453Triple-negative breast cancer (TNBC) is a breast cancer (BC) subtype that accounts for approximately 15–20% of all BC cases. Cancer cell lines (CLs) provide an efficient way to model the disease. We have recently isolated a patient-derived triple-negative BC CL MFUM-BrTNBC-1 and performed a detailed morphological and molecular characterisation and a comprehensive comparison with three commercial BC CLs (MCF-7, MDA-MB-231, MDA-MB-453). Light and fluorescence microscopy were used for morphological studies; immunocytochemical staining for hormone receptor, p53 and Ki67 status; RNA sequencing, qRT-PCR and STR analysis for molecular characterisation; and biomedical image analysis for comparative phenotypical analysis. The patient tissue-derived MFUMBrTNBC-1 maintained the primary triple-negative receptor status. STR analysis showed a stable and unique STR profile up to the 6th passage. MFUM-BrTNBC-1 expressed EMT transition markers and displayed changes in several cancer-related pathways (MAPK, Wnt and PI3K signalling; nucleotide excision repair; and SWI/SNF chromatin remodelling). Morphologically, MFUM-BrTNBC-1 differed from the commercial TNBC CL MDA-MB-231. The advantages of MFUM-BrTNBC-1 are its isolation from a primary tumour, rather than a metastatic site; good growth characteristics; phenotype identical to primary tissue; complete records of origin; a unique identifier; complete, unique STR profile; quantifiable morphological properties; and genetic stability up to (at least) the 6th passage.MDPI AG20222025-04-10 11:21:27Članek v reviji92461UDK: 618.19-006COBISS_ID: 91552259DOI: 10.3390/cells11010117ISSN pri članku: 2073-4409sl© 2021 by the authors