Chemo-enzymatic synthesis of viscoelastic pseudopeptidoglycan networksLoibner, Philipp (Avtor) Bučak-Gasser, David (Avtor) Schober, Katharina (Avtor) Steindorfer, Tobias Alexander (Avtor) Brandtner, Monika (Avtor) Dorn, Tobias (Avtor) Wrodnigg, Tanja (Avtor) Neshchadin, Dmytro (Avtor) Gescheidt-Demner, Georg (Avtor) Bračič, Matej (Avtor) Lackner, Florian (Avtor) Mohan, Tamilselvan (Avtor) Stana-Kleinschek, Karin (Avtor) Kargl, Rupert (Avtor) biomaterialsbacterial peptidoglycans (PGs)chemo-enzymatic synthesisBacterial peptidoglycans (PGs) are essential targets for antibiotics and immune cells. Chemical methods to reproduce PGs semisynthetically are tedious and wasteful. In this work, we describe a new approach to form pseudo-PGs (PPGs) using the protease papain and custom-made peptides conjugated to a glycan. The kinetics of formation is monitored by rheology and 1H NMR. Viscoelastic gels of controlled strength are formed, depending on the temperature and the number of peptide bridges between the glycan chains. We propose that the new method has a high impact on biomaterials research, since it could be used to deliver peptides, test antibiotic efficacy, or investigate human immune cell response.ACS Publications20252025-11-03 12:39:38Članek v reviji95867UDK: 576:60COBISS_ID: 254908163DOI: 10.1021/acs.bioconjchem.5c00014ISSN pri članku: 1520-4812sl