Protocol for the fabrication of self-standing (nano)cellulose-based 3D scaffolds for tissue engineeringMohan, Tamilselvan (Avtor) Bračič, Matej (Avtor) Bračič, Doris (Avtor) Lackner, Florian (Avtor) Nagaraj, Chandran (Avtor) Dobaj-Štiglic, Andreja (Avtor) Kargl, Rupert (Avtor) Stana-Kleinschek, Karin (Avtor) tissue engineering3D scaffoldsnanocellulosic materialsbiocompatibilityThree-dimensional (3D) and porous scaffolds made from nanocellulosic materials hold significant potential in tissue engineering (TE). Here, we present a protocol for fabricating self-standing (nano)cellulose-based 3D scaffolds designed for in vitro testing of cells from skin and cartilage tissues. We describe steps for preparation of nanocellulose ink, scaffold formation using 3D printing, and freeze-drying. We then detail post-processing procedures to enhance mechanical properties, stability, and biocompatibility. This protocol offers researchers a framework for developing versatile and sustainable biomaterials for regenerative medicine.Elsevier20252025-11-03 15:37:29Članek v reviji95871UDK: 677.46:620.3COBISS_ID: 254863619DOI: 10.1016/j.xpro.2024.103583ISSN pri članku: 2666-1667sl