20.500.12556/DKUM-100006 Priprava in karakterizacija akrilatnega polimera kot nosilca za imobilizacijo encima invertaze Preparation and characterisation of an acrylate polymer as a support for invertase enzyme immobilisation V okviru diplomske naloge smo pripravili visoko porozni akrilatni kopolimer na osnovi 2-hidroksietil metakrilata in N,N′-metilenbisakrilamida, ki smo ga uporabili kot nosilec za imobilizacijo encima. Kopolimer smo sintetizirali s prostoradikalsko polimerizacijo emulzije z visokim deležem notranje faze tipa olje v vodi. Kemijsko sestavo pripravljenega materiala smo proučili z infrardečo spektroskopijo s Fourierjevo transformacijo, s katero smo potrdili prisotnost strukturnih enot obeh monomerov v kopolimeru. Njegovo morfologijo in porozno strukturo smo preučili z vrstično elektronsko mikroskopijo. Pripravljeni kopolimer smo nato uporabili kot nosilec za imobilizacijo invertaze. Imobilizirani encim smo uporabili kot biokatalizator pri hidrolizi saharoze v glukozo in fruktozo. Reakcije smo izvedli pri temperaturah 30 in 35 °C ter pri različnih vrednostih pH pufra v območju od 3,5 do 5,0. Naraščanje koncentracije produktov hidrolize je pokazalo, da je invertaza po imobilizaciji na pripravljenem nosilcu ohranila katalitično aktivnost. Med preizkušenimi reakcijskimi pogoji smo najvišjo končno koncentracijo produktov dosegli pri 35 °C in pH 5,0. Dobljeni rezultati kažejo, da ima pripravljeni visoko porozni kopolimer na osnovi 2-hidroksietil metakrilata in N,N′-metilenbisakrilamida potencial za uporabo kot nosilec za imobilizacijo invertaze. As part of this thesis, a highly porous methacrylate copolymer based on 2-hydroxyethyl methacrylate and N,N′-methylenebisacrylamide was prepared and used as a support for enzyme immobilisation. The copolymer was synthesised by free radical polymerisation of an oil-in-water high internal phase emulsion. The chemical composition of the prepared material was investigated using Fourier transform infrared spectroscopy, which confirmed the presence of structural units derived from both monomers in the copolymer. Its morphology and porous structure were examined using scanning electron microscopy. The prepared copolymer was subsequently used as a support for invertase immobilisation. The immobilised enzyme was employed as a biocatalyst for the hydrolysis of sucrose into glucose and fructose. The reactions were carried out at 30 and 35 °C and at buffer pH values ranging from 3.5 to 5.0. The increasing concentrations of hydrolysis products demonstrated that invertase retained its catalytic activity after immobilisation on the prepared support. Among the reaction conditions tested, the highest final product concentration was obtained at 35 °C and pH 5,0. The results indicate that the prepared highly porous copolymer based on 2-hydroxyethyl methacrylate and N,N′-methylenebisacrylamide has potential for use as a support for invertase immobilisation. 2-hidroksietil metakrilat N N′-metilenbisakrilamida poliHIPE porozni polimer invertaza imobilizacija encima hidroliza saharoze. 2-hydroxyethyl methacrylate N N′-methylenebisacrylamide polyHIPE porous polymer invertase enzyme immobilisation sucrose hydrolysis true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-01 23:39:28 2026-09-22 16:13:54 2026-09-23 03:08:37 0000-00-00 00:00:00 2026 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 VS_Simonic_Nusa_2026.pdf VS_Simonic_Nusa_2026.pdf 1 D93BEFACF9A55C2ECD139E633289EE71 bb03239819f6fb7aa6c5a470d542e9a4c474222ce5a951dc2d00f5d8a75668a0 41db64c3-b68b-11f1-9ea3-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=213168 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0