20.500.12556/DKUM-100228 Optimizacija sinteze magnetnih nanodelcev železovega oksida z mikrovalovno metodo za imobilizacijo encimov Optimization of the microwave-assisted synthesis of iron oxide magnetic nanoparticles for enzyme immobilization Magnetni nanodelci železovega oksida so, zaradi svojih magnetnih lastnosti, velike specifične površine, primernosti za uporabo v bioloških sistemih in možnosti modifikacije njihove površine, pomemben material za uporabo na številnih področjih biotehnologije in biomedicine. Njihova površina zagotavlja mesta za vezavo različnih biomolekul, med drugim tudi encimov. Z vezavo encimov na magnetne nanodelce je omogočeno njihovo enostavno ločevanje iz reakcijskega medija z uporabo zunanjega magnetnega polja in predstavlja možnost za razvoj učinkovitejših biokatalitskih sistemov z možnostjo ponovne uporabe. Za uspešno uporabo je potrebna ustrezna priprava in funkcionalizacija nanodelcev ter izbira pogojev imobilizacije, ki omogočajo učinkovito vezavo encima ob ohranitvi njegove aktivnosti. Mikrovalovna sinteza predstavlja perspektiven pristop z vidika zelene kemije, saj lahko zaradi hitrega in učinkovitega segrevanja skrajša reakcijski čas ter zmanjša porabo energije. Diplomsko delo obsega sintezo magnetnih nanodelcev železovega oksida z mikrovalovno metodo in njihovo funkcionalizacijo s citronsko kislino. Lastnosti nastalih nanodelcev smo ovrednotili s termogravimetrično analizo, dinamičnim sipanjem svetlobe in Fourierjevo transformirano infrardečo spektroskopijo, s čimer smo spremljali njihove površinske značilnosti, hidrodinamsko velikost in izoelektrično točko. Na funkcionalizirane magnetne nanodelce smo z uporabo glutaraldehida imobilizirali encim lakazo. Pri optimizaciji smo proučevali vpliv koncentracij glutaraldehida in encima ter mase dobljenih delcev na uspešnost imobilizacije, ki smo jo vrednotili z Bradfordovo metodo in aktivnostnim testom. Rezultati nakazujejo vpliv izbranih pogojev na aktivnost lakaze in uspešnost imobilizacije. Due to their magnetic properties, high specific surface area, suitability for use in biological systems and the possibility for modifying their surface, magnetic iron oxide nanoparticles represent an important material for various applications in biotechnology and biomedicine. Their surface enables the binding of various biomolecules, including enzymes. The immobilization of enzymes on magnetic nanoparticles enables their easy separation from the reaction medium using an external magnetic field and offers potential for the development of more efficient and reusable biocatalytic systems. Successful application requires appropriate preparation and functionalization of the nanoparticles, as well as the selection of immobilization conditions that allow effective enzyme binding whilst preserving its activity. Microwave-assisted synthesis represents a promising approach from the perspective of green chemistry, as its rapid and efficient heating can shorten reaction times and reduce energy consumption. The thesis comprises the synthesis of magnetic iron oxide nanoparticles using a microwave assisted method and their functionalization with citric acid. The resulting nanoparticles were characterized by thermogravimetric analysis, dynamic light scattering, and Fourier transform infrared spectroscopy to investigate their surface properties, hydrodinamic size and isoelectric point. The functionalized magnetic nanoparticles were then used as carriers for the immobilization of the enzyme laccase using glutaraldehyde. During the optimization process, we investigated the effects of glutaraldehyde and enzyme concentrations, as well as the mass of the obtained nanoparticles, on the success of immobilization, which was assessed using the Bradford assay and enzyme activity assay. The results suggest that the selected conditions affect the activity of laccase and the immobilizzation efficiency. magnetni nanodelci železovega oksida mikrovalovna sinteza funkcionalizacija citronska kislina imobilizacija encimov lakaza iron oxide magnetic nanoparticles microwave-assisted synthesis functionalization citric acid enzyme immobilization laccase true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-09 11:32:08 2026-09-28 08:17:20 2026-09-28 08:17:22 0000-00-00 00:00:00 2026 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 UN_Kovacic_Petek_Nika_2026.pdf UN_Kovacic_Petek_Nika_2026.pdf 1 1137968D77D9093FD12DBF6760153DA3 006264b3adf2495c35506eae8e520f53ea631ba25af0d90711a279528502bb58 a3ff4128-ad38-11f1-b668-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=212130 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0