20.500.12556/DKUM-100390 Strain- and matrix-dependent survival of Lactobacillus acidophilus LA-5 and Lacticaseibacillus paracasei 431 in commercial ice cream bases Od seva in matriksa odvisno preživetje Lactobacillus acidophilus LA-5 in Lacticaseibacillus paracasei 431 v komercialnih osnovah za sladoled The survival of Lactobacillus acidophilus LA-5 and Lacticaseibacillus paracasei 431 was evaluated in commercially available ice cream base mixtures differing in dairy and plant-based fat content. The objective was to determine strain-dependent stability during frozen storage and to model viability loss using the Weibull inactivation equation. Following inoculation, samples were frozen and stored at −20 °C for 90 days. Viable counts were monitored periodically, and survival kinetics were described by non-log linear regression. A significant reduction in cell numbers was observed after a single freeze–thaw cycle in both strains. During subsequent storage, gradual inactivation occurred, with distinct strain- and matrix-dependent differences. L. acidophilus LA-5 showed higher stability in formulations with dairy-based fat compared with the plant-based fat, whereas L. paracasei 431 exhibited shorter predicted times to equivalent log-reductions in dairy systems. Survival curves were adequately described by the Weibull model, indicating heterogeneous resistance within bacterial populations. The results demonstrate marked strain- and formulation specific differences in probiotic stability during frozen storage and provide quantitative parameters for predicting viability loss in ice cream matrices. ice-cream probiotics Lactobacillus acidophilus Lacticaseibacillus paracasei frozen storage sladoled probiotiki Lactobacillus acidophilus Lacticaseibacillus paracase zamrznjeno skladiščenje true true true Angleški jezik Slovenski jezik Znanstveno delo 2026-09-16 10:17:10 2026-09-16 10:17:11 2026-09-17 03:10:57 0000-00-00 00:00:00 2026 0 0 str. 1-7 No. 1 Vol. 23 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-07-14 579.6 285239555 10.18690/agricsci.23.1.1 2820-610X https://journals.um.si/index.php/agricultura/en/article/view/6244 1 086642a3-b1a7-11f1-b05b-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=212565 RAZ_Prijatelj_Kristjan_2026.pdf RAZ_Prijatelj_Kristjan_2026.pdf 1 A6299AD49E3F0AE61CA86CFDBBE28100 39ceb2b2d160cb3709327796b4b64365f30a0586ca60378f042fbcd42df12408 133a943b-b1a8-11f1-b05b-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=212566 Fakulteta za kmetijstvo in biosistemske vede Agricultura scientia 0 0 0