20.500.12556/DKUM-10897 SEPARACIJA BIOAKTIVNIH KOMPONENT IZ BRUSNIC SEPARATION OF BIOACTIVE COMPOUNDS FROM CRANBERRIES S pomočjo konvencionalne ekstrakcije in superkritične ekstrakcije s CO2 posušenih plodov brusnic smo pripravili ekstrakte v katerih smo določili vsebnost nekaterih fenolnih spojin. Pri konvencionalni ekstrakciji smo kot topilo uporabili 60 % raztopino acetona in 35 % raztopino etanola. Ekstrakcije smo izvajali pri temperaturi 22 °C in pri temperaturi 40 °C v dveh stopnjah. V pripravljenih ekstraktih smo določali koncentracije celokupnih monomernih antocianinov, celokupnih fenolov, celokupnih flavonoidov, proantocianidinov in taninov s spektrofotometričnimi metodami. Merili smo jim absorbanco pri različnih valovnih dolžinah in na podlagi dobljenih umeritvenih krivulj izračunali koncentracije. Rezultate smo podali na gram ekstrakta in na gram materiala. Prav tako smo spremljali kinetiko ekstrakcije, tako da smo med postopkom ekstrakcije jemali vzorce v različnih časovnih intervalih in spremljali spreminjanje koncentracije v odvisnosti od časa. Dried cranberries were extracted and obtained extracts were analyzed on the content of phenolic compounds. Conventional extraction with organic solvents and supercritical extraction with CO2 were applied. For conventional extraction 60 % solution of acetone and 35 % solution of ethanol were used. Extractions were performed at temperature 22°C and at temperature 40 °C in two steps. Concentrations of total monomeric anthocyanins, total phenols, total flavonoids, proanthocyanidins and tanins were determined in prepared extracts by spectrophotometric methods. The absorbances at different wavelengths were measured and according to calibration curves concentrations were calculated. Results were expressed per gram of extract and per gram of material. The kinetics of extractions was followed, so that the samples were taken at different time intervals during the process of extraction and variation of concentration with time was monitored. Konvencionalna ekstrakcija superkritična ekstrakcija s CO2 brusnica celokupni fenoli celokupni flavonoidi celokupni antocianini tanini proantocianidini kinetika ekstrakcije Conventional extraction supercritical extraction with CO2 cranberry total phenols total flavonoids total anthocianins tanins proanthocianins kinetics of extraction true true false [M. Peklar] Slovenski jezik Angleški jezik Diplomsko delo 2009-06-13 11:22:08 2009-12-22 09:45:34 2022-04-11 16:39:30 2022-04-17 03:18:26 2009 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.061.34:547.568(043.2) 13440022 URN:SI:UM:DK:SXAYGCT9 UNI_Peklar_Mojca_2009.pdf UNI_Peklar_Mojca_2009.pdf 1 B87111C648E5D6952AF71C413A755376 8e59bcb0fd29cbe45de68a7be3d3f0a34d1014df503dcd92196d2e0d4697c85e f583e2ae-7c02-11eb-bb7a-00155d0001ca 20.500.12556/dkum/b54dd320-abca-4d8c-91c0-22384d778b84 https://dk.um.si/Dokument.php?lang=slv&id=9283 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0