20.500.12556/DKUM-12234 IZOLACIJA IN KARAKTERIZACIJA BAKTERIJ IZ RIZOPLANA HLADNIKIJE Isolation and characterization of bacteria from Rhizoplane of Hladnikia pastinacifolia Na površini korenin, imenovani rizoplan, lahko najdemo zelo različne skupine mikroorganizmov. Ti lahko na rast rastlin vplivajo pozitivno, lahko pa s svojo prisotnostjo in delovanjem rastlini škodujejo. Cilj diplomskega dela je bil izolacija in identifikacija bakterij, ki naseljujejo rizoplan hladnikije — slovenske endemične vrste. Iz treh rastlin smo osamili 17 bakterijskih sevov. Izolirali smo bakterijsko DNA in s pomočjo verižne reakcije s polimerazo (PCR) pomnožili 16S rDNA. Glede na primerjavo pomnožene 16S rDNa z referenčnimi bazami podatkov (GenBank, Ribosomal database project) smo izolirane bakterije identificirali. Izolirane bakterije so pripadale rodovom Bacillus, Enterobacter, Pseudomonas, Erwinia in Pantoea. On the surface of roots called rhizoplane various groups of microorganisms are present. They can have plant growth promoting effects, but their presence and activity can also have inhibit the plant growth. The aim of the thesis was the isolation and identification of bacteria, which dwell in the rhizoplane of the Slovene endemic genus Hladnikia. From three plants we isolated17 bacterial strains. We isolated the DNA from the bacteria and amplified 16S rDNA regions with polymerase chain reaction (PCR); The identification of the strains was done by compariosn of 16S rDNa sequences with reference databases GenBank and Ribosomal Database Project. The isolated bacteria belonged to the genera Bacillus, Enterobacter, Pseudomonas, Erwinia and Pantoea. Hladnikija Hladnikia pastinacifolia 16S rDNA PCR verižna reakcija s polimerazo rizosfera rizoplan rizobakterije Hladnikia pastinacifolia 16S rDNA PCR polymerase chain reaction rhizospere rhizoplane rhizobacteria true true false [T. Mithans] Slovenski jezik Angleški jezik Diplomsko delo 2009-10-19 08:52:34 2009-11-25 15:48:05 2022-04-12 07:19:53 2022-04-19 03:17:50 2009 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57/59(043.2) 17242632 URN:SI:UM:DK:DKOILT6K UNI_Mithans_Tamara_2009.pdf UNI_Mithans_Tamara_2009.pdf 1 094F11CB0B384BF68BC60BB235847A90 5018f6649e8ee2427d8880ccd8efc8a71ed8a72b567a6dbf9c8a48da03c9ef33 5a48d5bc-7c03-11eb-bb7a-00155d0001ca 20.500.12556/dkum/d5be87e2-d3a1-4786-969e-87c7e6e1be2d https://dk.um.si/Dokument.php?lang=slv&id=11478 Fakulteta za naravoslovje in matematiko 0 0 0