20.500.12556/DKUM-14015 BIOLOŠKA PREDELAVA ODPADNE SULFITNE TEKOČINE ZA PROIZVODNJO BIOETANOLA IN ENOCELIČNIH PROTEINOV BIOPROCESSING OF SPENT SULPHITE LIQUOR FOR BIOETHANOL AND SINGLE CELL PROTEIN Odpadna sulfitna tekočina iz trdega lesa je odpadni stranski proizvod industrije celuloze in papirja, ki proizvaja beljeno papirno kašo s sulfitnim postopkom, iz lesa Eucalyptus globulus. Po sulfitnem postopku odpadna sulfitna tekočina izhlapi in se sežge za povračilo energije. Glavni sestavni deli sulfitne tekočine so predvsem sulfatni lignin in sladkorji, iz degradirane hemiceluloze, predvsem pentoze. Ksiloza je glavni predstavnik pentoze in vsebuje tudi ocetno kislino, ki je zaviralec kvasovk. Odpadna sulfitna tekočina se lahko uporablja za proizvodnjo izdelkov z dodano vrednostjo. Obstajajo različne uporabe enoceličnih proteinov za človeško prehrano in živalsko krmo, prispevajo k zmanjšanju vrzeli v povpraševanju in dobavo hrane v svetu. Bioetanol se lahko uporablja kot obnovljivi vir tekočih goriv za motorna vozila. Uporablja se tudi v kozmetični, farmacevtski in prehrambeni industriji. Namen te študije je bil uporaba odpadne sulfitne tekočine za proizvodnjo enoceličnih proteinov z vlaknastimi glivami Paecilomyces variotii in za pospeševanje fermentacijskih postopkov s Pichia stipitis z razstrupljeno odpadno sulfitno tekočino trdega lesa za pridobitev bioetanola. Prvi del tega dela je bil študij fermentacije s Paecilomyces variotii v odpadni sulfitni tekočini. S fermentacijo gliv je bila dosežena razstrupljena odpadna sulfitna tekočina. Ta fermentacija je bila izvršena v enostavnem šaržnem in zaporednem šaržnem reaktorju. Fermentacija gliv v zaporednem šaržnem reaktorju je bila narejena v dveh in treh zaporednih šaržnih reaktorjih, kjer so bile šarže povezane z prejšnjo šaržo. Zaporedni šaržni reaktor z dvema zaporednima šaržama je bila boljša strategija za pridobivanje razstrupljene odpadne sulfitne tekočine kot enostavna šarža. Biomasa gliv ima 65 % vsebnost beljakovin in vsebuje 8,2 % ± 0,2 nukleinskih kislin. Drugi del tega dela vključuje fermentacijo s Pichia stipitis. V teh postopkih fermentacije je bila razstrupljena odpadna sulfitna tekočina fermentirana s Pichia stipitis za proizvodnjo etanola. Donos etanola je bil 0,015 g etanol/g biomase h. Največja produktivnost etanola je bila 0,085 g etanol/L h. Koncentracija sladkorjev, ocetne kisline in etanola je bila merjena s HPLC. Papirna industrija vključena v tem sodelovanju je bila Caima, SA. The Hardwood Spent Sulfite Liquor (HSSL) is the waste by-product of the pulp and paper industries that produce bleached pulps by acidic sulphite pulping, of Eucalyptus globulus wood. After the pulping, HSSL is evaporated and then burned for energy recovering. HSSL contains essentially sulphonated lignin (lignosulphonates) and sugars from degraded hemicelluloses, mainly pentoses. Xylose is the main pentose present and it also contains acetic acid that is a yeast inhibitor. HSSL can be used to produce added value products. There are different applications for Single Cell Protein (SCP) such as human food and animal feed that contribute for the reduction of the gap in demand and supply of food in the world. Bioethanol can be used as a renewable liquid fuel for motor vehicles. It is also used in cosmetic, pharmaceutical and food industry. The aim of this work was to study the use of HSSL for production of SCP with filamentous fungus Paecilomyces variotii and to promote Pichia stipitis fermentation processes of the detoxified HSSL in order to obtain bioethanol. The first part of this work was to study Paecilomyces variotii fermentation in HSSL. With fungus fermentation deacidificated liquor was obtained. This fermentation was made in simple batch and in sequential batch reactor (SBR). SBR fungus fermentation was made in two and three batches, where batches were connected with previous batch. SBR with two batches was a better strategy to obtain liquor deacidification than simple batch. Fungus biomass has 65 % content of protein and 8.2 % ± 0.2 of nucleic acids. The second part involved Pichis stipitis fermentation. In these fermentations the deacidificated liquor was fermented by P. stipitis to produce ethanol. Ethanol yield was 0.015 g ethanol/g biomass h. Maximum productivity of ethanol was 0.085 g ethanol/L h. Concentration of sugars, acetic acid and ethanol were accessed by HPLC. The paper industry involved in this cooperation is Caima, SA. Enocelični proteini bioetanol odpadna sulfitna tekočina fermentacija Paecilomyces variotii Pichia stipitis Single Cell Protein bioethanol sulfite spent liquor fermentation Paecilomyces variotii Pichia stipitis deacidification true true false [Š. Ivanuša] Slovenski jezik Angleški jezik Diplomsko delo 2010-05-19 11:36:13 2010-05-25 11:21:49 2022-04-12 11:39:57 2023-12-17 03:11:19 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 662.7:664.12(043.2) 14146838 URN:SI:UM:DK:AMCCWBKX UNI_Ivanusa_Spela_2010.pdf UNI_Ivanusa_Spela_2010.pdf 1 4E187AE06C2B7DE03B4FF728D26C3A4C 60871a2b052e11cdaf54c3946a3624c717b973bfd42a784cc5f4498d88041221 0d003fc1-7c04-11eb-bb7a-00155d0001ca 20.500.12556/dkum/3e11aa52-bf43-4d9a-ad07-99edcc63026f https://dk.um.si/Dokument.php?lang=slv&id=14640 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0