20.500.12556/DKUM-14411 Validacija analizne metode za določanje glukozinolatov v semenih navadnega rička (Camelina sativa ) Validation of analytical method for determination of glucosinolates in false flax seeds (Camelina sativa ) Namen diplomskega dela je bila validacija analizne metode za določanje glukozinolatov v semenih navadnega rička. Določili smo vsebnosti glukozinolatov z visokotlačno tekočinsko kromatografijo (po dveh različnih metodah) v 27 vzorcih slovenskega porekla, treh zaporednih letin (2007,2008 in 2009). Za primerjavo smo določili glukozinolate še 4 vzorcem iz tujine (Danska, Nemčija, ZDA in Finska). Vzorcem smo določili še delež vlage in količino olja. Opravili smo validacijo analizne metode, določili linearnost, natančnost, mejo določljivosti, časovno stabilnost ekstrakta in vpliv vremenskih razmer na vsebnost glukozinolatov. Na osnovi vseh rezultatov smo ugotovili, da gre v Sloveniji za eno samo vrsto navadnega rička, saj se nikjer med analiziranimi vzorci ne pojavljajo signifikantne razlike. Purpose of this thesis was the validation of the analytical method for the determination of glucosinolates in the seeds of false flax. We have determined the content of glucosinolates with high pressure liquid chromatography, using two different methods. We have used 27 samples of Slovenian origin from three consecutive harvests (2007, 2008 and 2009). For comparison, we have determined the glucosinolates content in 4 other samples. These samples originated from Denmark, Germany, USA and Finland. Furthermore, we have also determined the moisture content and the quantity of oil. We have performed complete validation of the analytical method. We have determined linearity, precision, limit of quantification, temporal stability of the extract and the impact of weather on the content of glucosinolates. Based on all final results, we have concluded that in Slovenia exists only one species of false flax, because there were no significant differences between the analyzed samples. navadni riček Camelina sativa glukozinolati HPLC validacija false flax Camelina sativa glucosinolates HPLC validation true true false [A. Moškon] Slovenski jezik Angleški jezik Diplomsko delo 2010-06-11 15:29:22 2010-07-20 12:37:27 2022-04-12 22:00:35 2023-12-17 03:28:07 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.85:543.544(043.2) 14345238 URN:SI:UM:DK:UHJDMKJ4 UNI_Moskon_Anja_2010.pdf UNI_Moskon_Anja_2010.pdf 1 06D12095D83ED046567A064E88C553D4 3b1e80b6961f4ab418024d4aea264e6a9972488076e64bb3c33be8b54778c5f1 24454295-7c04-11eb-bb7a-00155d0001ca 20.500.12556/dkum/3cf947bc-a9ea-402b-8b58-8ce2b713e7a0 https://dk.um.si/Dokument.php?lang=slv&id=15326 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0