20.500.12556/DKUM-16088 IZDELAVA IN KARAKTERIZACIJA HITOZANSKIH NANODELCEV ZA MEDICINSKO UPORABO PRODUCTION AND CHARACTERIZATION OF CHITOSAN NANOPARTICLES FOR MEDICAL USE Namen diplomskega dela je bil izdelati in okarakterizirati nanodelce, ki nastanejo s tehniko ionotropnega geliranja iz hitozana in natrijevega tripolifosfata (TPP). Nanodelcem smo določili naboj s polielektrolitsko titracijo, velikosti z metodo dinamičnega sipanja laserske svetlobe (DLS) in zeta potencial z elektroforetičnim sipanjem svetlobe ter protimikrobni učinek po standardu ASTM E 2149-01. Preverili smo vpliv različnih masnih razmerij reaktantov in vpliv dodatka stabilizatorja polietilen glikola (PEG) na lastnosti disperzije nanodelcev. Ugotovili smo, da se s povečanjem masnega razmerja hitozan/TPP povečujejo: množina prostih amino skupin in s tem naboj, hidrodinamski radij ter zeta potencial, kakor tudi protimikrobni učinek na nekatere testirane mikroorganizme. Ugotovili smo, da dodatek stabilizatorja polietilen glikola (PEG) k disperziji nanodelcev ne vpliva izrazito na spremembe hidrodinamskega premera nanodelcev, medtem ko znižuje heterodisperznost velikosti nanodelcev in zeta potencial, in tako naredi disperzije manj stabilne. Iz tega sklepamo, da PEG proučevanih molekulskih mas ni najustreznejši stabilizator za disperzije hitozanskih nanodelcev. Ugotovili smo, da so nanodelci protimikrobno učinkoviti; presenetljivo celo bolje inhibirajo rast mikroorganizmov kot sama raztopina hitozana. Nano-formulacija hitozana omogoča njegovo optimalno in nadzorovano difuzijo v celice patogenih mikroorganizomov in je tako njihova degradacije še učinkovitejša. In this study, we tried to obtain nanoparticles from chitosan and sodium tripolyphosphate (TPP) using ionotropic gelation techniques. The obtained nanoparticles were characterized for positive charge using polyelectrolyte titrations, particle size using dynamic light scattering (DLS), zeta potential of particles using electrophoretic light scattering, as well as antibacterial activities of nanoparticles were examined using standard ASTM E 2149-01. The results show that an increase in the w/w ratio of chitosan/TPP triggers an increase in the amount of free amino groups (positive charge), hydrodynamic diameter, zeta potential and also intensifies the antimicrobial effect of nanoparticles. The results also show that the addition of stabilizer polyethylene glycol (PEG) does not have major effect on the hydrodynamic diameter of nanoparticles, however it decreases heterodispersity of the size as well zeta potential, thus making dispersions less stable. This leads to the conclusion that PEG is not an appropriate stabilizer for studied nanoparticles dispersions. Moreover, the antibacterial activity results suggests that the chitosan in nanoparticulate form display higher antibacterial activity against all tested microorganisms than the chitosan in the free form. Nano-formulation of chitosan enables optimal and controlled diffusion of chitosan into the cells of pathogen microorganisms, thus making their degradation more efficient. hitozan nanodelci TPP ionotropno geliranje ionsko geliranje protimikrobna učinkovitost chitosan nanoparticles TPP ionotropic gelation ionic gelation antimicrobial activity true true false [M. Kolar] Slovenski jezik Angleški jezik Diplomsko delo 2010-09-30 09:44:39 2010-09-30 14:30:22 2022-04-13 07:51:35 2023-12-20 03:16:35 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.15:66.098:620.3(043.2) 14560278 URN:SI:UM:DK:YRTTXPAX UNI_Kolar_Metod_2010.pdf UNI_Kolar_Metod_2010.pdf 1 910F743495561CDC8969056263F691C1 6efa64d54140df22f358c290fd68531bdb3c4a36488b16b8a2106097a5560133 8fce7454-7c04-11eb-bb7a-00155d0001ca 20.500.12556/dkum/15fb2da3-63c2-4683-a648-a408bb591f8d https://dk.um.si/Dokument.php?lang=slv&id=18046 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0