20.500.12556/DKUM-17052 Lokalni odziv skupnih proteinov v lubju navadne smreke na tretiranje s salicilno kislino in napad podlubnikov The local protein response in the phloem of Norway spruce after salicylic acid treatment and bark beetle attack Osmerozobi smrekov lubadar (Ips typographus L.) je najpomembnejši škodljivec navadne smreke v Evropi. Na leto se zaradi napada podlubnikov v Sloveniji v povprečju poseka 350000 m3 lesa. Njegovo aktivnost smo spremljali skozi celotno vegetacijsko dobo leta 2008 in določili dva maksimalna naleta konec maja in konec julija, kar sovpada z visokimi temperaturami in sušnim obdobjem. V drugem sklopu poskusa smo odseke debel navadne smreke tretirali s 100 mM salicilno kislino, ki je kot signalna molekula sposobna inicirati gene za sintezo obrambnih proteinov. Preučili smo vpliv tretiranja s salicilno kislino na vsebnost skupnih proteinov v sekundarnem floemu navadne smreke od inicialnega napada do uspešne naselitve podlubnikov. Pri prvem vzorčenju (13. 4. 2007) smo pri tretiranih drevesih izmerili nižjo vsebnost skupnih proteinov v primerjavi z netretiranimi kontrolnimi drevesi, medtem ko smo sredi maja določili 97,5 % porast proteinov v lubju tretiranih kontrolnih dreves v primerjavi z netretiranimi drevesi. Pri tretjem vzorčenju (15. 6. 2007) smo določili kvantitativne razlike v obrambnem odzivu skupnih proteinov zmerno napadenih dreves in močno napadenih dreves. V močno napadenih drevesih je vsebnost skupnih proteinov padla, vendar so bile višje vrednosti pri tretiranih, močno napadenih drevesih v primerjavi z netretiranimi. Pri zmerno napadenih drevesih smo zaznali akumulacijo skupnih proteinov v lubju. Najvišjo vsebnost skupnih proteinov smo izmerili pri tretiranih, zmerno napadenih, drevesih, kar nakazuje, da je tretiranje s salicilno kislino vzpodbudilo uspešen obrambni odziv dreves in zavrlo naselitev podlubnikov. The eight-toothed spruce bark beetle (lps typographus L.) is the most important pest of Norway spruce in Europe. Due to bark beetle attack,close to 350000 m3 timber is felled annually in Slovenia. Its activity was monitored through the entire vegetation period in 2008 and two maximum attacks were identified at the end of May and July, which coincided with high temperatures and dry season. Stem sections of the Norway spruce were treated with 100 mM salicylic acid in the second part of the experiment, in order to examine its impact on total protein contents in the secondary phloem from initial attack to successful colonization of bark beetles. Salicylic acid as a signaling molecule is able to induce the expressions of many defense-related genes for the synthesis of defense proteins through different pathways. At the first sampling date (13.4.2007), we measured a lower amount of total proteins in treated trees when compared to untreated control ones, while we noted the increase of proteins by 97, 5 % in the bark of treated control trees compared to the untreated ones in the middle of May. On 15 June 2007, quantitative differences in protein response were determined between moderately and strongly infested trees. In massively infested trees, the total protein contents dropped, but the values were higher than in the treated strongly infested trees compared with the untreated ones. The highest amount of total proteins was measured in treated moderately infested trees, suggesting that treatment with salicylic acid stimulated a successful defense response and inhibited the colonization of bark beetles. salicilna kislina skupni proteini navadna smreka [Picea abies (L.) H. Karst] osmerozobi smrekov lubadar (Ips typographus L.) aktivnost podlubnikov salicylic acid total proteins Norway spruce [Picea abies (L.) H. Karst] eight-toothed spruce bark beetle (Ips typographus L.) bark beetle activity period. true true false Slovenski jezik Angleški jezik Diplomsko delo 2010-12-16 17:38:54 2011-01-11 10:21:49 2022-04-13 09:09:12 2023-12-21 03:31:04 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:RRXNK4VD UNI_Gjergjek_Robi_2010.pdf UNI_Gjergjek_Robi_2010.pdf 1 A53CA1C51B3E41B29CE6B636BA23A8FB b7ed0909e0c5b99f80b7039d7041190579fad2fa1bb12e2d795af8b906407d32 cbf16d9a-7c04-11eb-bb7a-00155d0001ca 20.500.12556/dkum/9149c7ce-87df-4148-ad07-36308c871cf0 https://dk.um.si/Dokument.php?lang=slv&id=19659 Fakulteta za kmetijstvo in biosistemske vede 0 0 0