20.500.12556/DKUM-17907 RAZŠIRJENOST RH FENOTIPA KRVODAJALCEV V SEVEROVZHODNI SLOVENIJI THE FREQUENCY OF THE BLOOD DONORS RH FENOTYPE IN THE NORTHEASTERN PART OF SLOVENIA Pri transfuziji, oziroma zdravljenju s krvjo, je eden najtežjih zapletov hemoliza transfundiranih eritrocitov. V vecini primerov jo lahko preprecimo z zagotavljanjem skladne krvi. Najpomembnejša informacija pri tem postopku je poleg podatkov o krvni skupini AB0, RhD in Kell antigenu, še podatek o prisotnost i klinicno pomembnih protiteles (sistem Rh, MNSs, P, Le, Lu, K, Fy, Jk in drugi) v serumu bolnika. Bolnik, ki ima v serumu prisotna protitelesa (PT) proti dolocenim antigenom(Ag), bo prejel kri, oziroma eritrocite krvodajalca, brez teh antigenov. Zaradi hitrejšega in lažjega iskanja skladne krvi za bolnika pri vsakem krvodajalcu opravimo 4 osnovne teste: 1. dolocitev krvne skupine AB0 in RhD antigena na er itrocitih, 2. Kell antigena na eritrocit ih, 3. Rh fenotipa (C,c,E in e antigen) na er itrocitih in 4. Indirektni Coombsov test – ICT (prisotnost protiteles v serumu). Vse rezultate povežemo in shranimo v racunalniški informacijski sistem DATEC in tako na najhitrejši nacin poišcemo kri oziroma eritrocite krvodajalca, ki bodo najbolj ustrezale bolniku. Namen našega dela je, da s pomocjo laboratorijskega aparata Maestro Jazz in Saxo (DiaMed, Švica), pri cemer bomo uporabljali aglutinacijsko tehniko, dolocimo prisotnost Rh fenotipa na eritrocitih krvodajalca in ugotovimo razširjenost le tega v severovzhodnem delu Slovenije. Opravili bomo 3000 testiranj Rh fenotipa, za 1000 krvodajalcev na obmocju Maribora, 1000 krvodajalcev na obmocju Ptuja in 1000 krvodajalcev na obmocju Murske Sobote ter raziskali pogostost posameznih fenotipov na dolocenem obmocju. Rezultate bomo nato povezali z obstojecim hišnim informacijskim sistemom DATEC tako, da bomo lahko za bolnika na cim hitrejši nacin prišli do ustrezne krvi oziroma eritrocitov krvodajalca, ki ima v serumu prisotna dolocena protitelesa. One of the major complications during the blood transfusion or during the blood healing process is the hemolysis of the transfused erythrocytes. In the majority of the cases it can be prevented by providing adequate blood. Besides the data relative to the blood groups AB0, RhD and Kell antigene the most important information regarding this procedure is the information about the presence of clinically important antibodies (the Rh system, MNSsP, Le, Lu, K, Fy, Jk and others) in the serum of the patient. The patient, in whose serum are present antibodies (PT) against antigenes (Ag) will accept blood, that is erythrocytes of the blood donor without these ant igenes. To achieve a faster and easier locating of the appropr iate blood for the patient, it is advisable to perform 4 basic test of the donor's blood: 1. Def init ion of the blood groups AB0 and RhD antigene on the erythrocytes, 2. Kell antigene on the erythrocytes, 3. Rh fenotype (C,c,E and e antigene) on the erythrocytes and 4. Indirect Coombs Test – ICT (presence of antibodies in the serum). All the results will be subsequent ly processed and stored in the computer information system DATEC: this is the fastest way to f ind the needed blood i.e the erythrocytes of the donor, that are most suitable for the pat ient. The scope of our work is to determine the Rh fenotype on the erythrocytes of the patient and to assess his f requency in the northeastern part of Slovenia. The research work will be carr ied out by means of the laboratory apparatus Maestro Jazz and Saxo (DiaMed, Switzerland). We will perform 3000 tests of the Rh fenotype on 1000 blood donors in the Mar ibor area, on 1000 blood donors in the area of Ptuj and on 1000 blood donors in the area of Murska Sobota and we will determine the f requency of any part icular fenotype in a certain area. We will compare and process the results with the existent information in our home system DATEC and we will reach the appropr iate blood i.e. the erythrocytes of the blood donor for the patient, in whose serum certain antibodies are present. Cloveška kri Vsebnost antigenov na er itrocitih krvodajalcev krvna skupina Rh fenotip Kell antigen Human blood Ant igen content in erythrocytes of blood donors Blood group Rh phenotype Kell Antigen true true false [A. Cvetko] Slovenski jezik Angleški jezik Diplomsko delo 2011-03-29 11:29:08 2011-03-30 09:00:28 2022-04-13 10:15:40 2023-12-23 03:10:41 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 661-097.1(043.2) 15004694 URN:SI:UM:DK:DR2M4PQB VS_Cvetko_Andreja_2011.pdf VS_Cvetko_Andreja_2011.pdf 1 174990D3ADB5C15FCA90E7D7BD297784 30528d164520a9f08a36bf3553ac2e514cb61c797eedf877dd938c40e53e57eb 0083a675-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/adb54a89-2dad-4966-8991-b2fb6aee8cbc https://dk.um.si/Dokument.php?lang=slv&id=21125 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0