20.500.12556/DKUM-18171 LOČEVANJE LIPIDNIH RAZREDOV S TANKOPLASTNO KROMATOGRAFIJO IN EKSTRAKCIJO NA TRDNI FAZI SEPARATION OF LIPID CLASS WITH THIN LAYER CHROMATOGRAPHY AND SOLID PHASE EXTRACTION Za ločevanje lipidov v njihove razrede smo uporabili metodo tankoplastne kromatografije (TLC) in jo po optimizaciji prenesli na metodo ločevanja na trdni fazi (SPE). Vse teste smo izvedli na standardnih spojinah najbolj značilnih predstavnikov lipidov. Rezultate smo ovrednotili na podlagi primerjave kromatogramov, ki smo jih dobili s preizkusi nekaj kombinacij razvijalnih raztopin na TLC in adsorpcijskih faz na SPE. Za našo metodo sta najbolj primerni razvijalni raztopini heksan : dietileter (65 : 35 (v/v)) in kloroform : metanol:voda (65 : 25:4 (v/v/v)), razvijalni raztopini kloroform : metanol:ocetna kislina (100 : 5 : 2 (v/v/v)) in heksan :dietileter : metanol : ocetna kislina (90 : 20 : 5 : 2 (v/v/v)) sta zaradi slabše ločljivosti lipidov manj primerni. Iz rezultatov je razvidno, da je za našo metodo najprimernejša kolona s silikagel polnilom proizvajalca Varian, ki jo kondicioniramo s kloroformom in metanolom. Aminske SPE kolone različnih proizvajalcev niso bile primerne. Uspešni rezultati testov ponovljivosti in obnovljivosti metode so pokazali, da je uporabljena analizna metoda primerna za ločevanje izbranih lipidov v posamezne lipidne razrede. Thin layer chromatography (TLC) and solid phase extraction (SPE) were used for separation of lipid classes. After optimization the thin layer chromatographic method was transferred to solid phase extraction (SPE) using standard solutions of lipids. Results were evaluated by comparison of chromatograms obtained with experiments with some possible combination of solutions on TLC and SPE absorption phases. Most suitable developing solutions are hexane:ether (65:35 (v/v)) and chloroform: methanol:water (65:25:4 (v/v/v)), while solutions chloroform:methanol:acetic acid (100:5:2 (v/v/v)) and hexane ether:methanol:acetic acid (90:20:5:2 (v/v/v)) are less appropriate owing of unsuccesful separation. From results it is evident that the most appropriate column is Si – column brand Varian, conditioned with chloroform and methanol. NH2 columns from different manufacturers are not appropriate. Successful results of test repeatability, reproducibility and accuracy of method showed, that applied analytical method is appropriate for lipid separation into individual lipid classes. lipidi fosfolipidi ekstrakcija tekoče-tekoče tankoplastna kromatografija (TLC) ekstrakcija na trdni fazi (SPE). Lipids Phospholipids liquid-liquid extraction thin layer chromatography (TLC) Solid – phase extraction (SPE). true true false [D. Al Mahdawi] Slovenski jezik Angleški jezik Diplomsko delo 2011-04-22 21:55:56 2011-05-03 09:30:23 2022-04-13 10:29:52 2023-12-22 03:44:06 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.9(043.2) 14977302 URN:SI:UM:DK:0UYFAHYY UNI_Al_Mahdawi_Dunja_2011.pdf UNI_Al_Mahdawi_Dunja_2011.pdf 1 2A2522F97B2A3901F164C3D946581FFD 188dd4024a92e9212e39daaffd9df7bb535ea9a159ef244527ca8fe44250b72f 103e1917-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/c7bad944-2edf-482a-8f9a-8557ff203117 https://dk.um.si/Dokument.php?lang=slv&id=21583 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0