20.500.12556/DKUM-18928 Vpliv jasmonske kisline, ranitve in organsko revnega gojišča na regeneracijo listov praproti Platycerium bifurcatum (Cav.) C. Chr. v tkivni kulturi The effect of jasmonic acid, wounding and organically deficient medium on regeneration of Platycerium bifurcatum (Cav.) C. Chr. fern leaves in tissue culture Raziskali smo vpliv jasmonske kisline (JA) in ranitve ter sočasni učinek obeh dejavnikov na regeneracijo listov praproti Platycerium bifurcatum v tkivni kulturi. V MS gojišče z 0,01% saharoze ter z 0, 0.1, 1, 10 in 100 µM JA smo polagali neranjene ločene liste ter ločene liste, ki smo jih različno močno ranili. Po 50 dneh kulture smo na listih spremljali razvoj novih struktur: brstov, rizoidov, apospornih gametofitov in intermediatov. Ugotovili smo, da nižje koncentracije JA pospešujejo razvoj brstov, rizoidov ter intermediatov, medtem ko višje koncentracije vzpodbudijo razvoj apospornih gametofitov. Na listih z ranjeno zgornjo povrhnjico se je razvilo največje število sporofitskih struktur (brstov in rizoidov) ter intermediatov, medtem ko je bil razvoj apospornih gametofitov najbolj pospešen na nasekljanih listih. Regeneracija listne kulture pod vplivom različnih koncentracij eksogeno dodane JA je bila podobna regeneraciji, sproženi kot odziv na različne stopnje ranitve. The effect of jasmonic acid (JA), wounding and simultaneous influence of both parameters on regeneration of Platycerium bifurcatum fern leaves in tissue culture were studied. Intact separate leaves and separate leaves that were wounded to varying degrees were placed flat on MS medium supplemented with 0.01% sucrose and with 0, 0.1, 1, 10 in 100 µM JA. Af-ter 50 days of culture new structures developed on leaves: buds, rhizoids, aposporous game-tophytes and intermediates. We found out that low JA concentrations promote development of buds, rhizoids and intermediates, while higher concentrations stimulate development of aposporous gametophytes. The largest number of sporofitic structures (buds and rhizoids) and intermediates developed on leaves with cutted adaxial epidermis, while development of aposporus gametophytes was largely accelerated on chopped leaves. Regeneration of leaf culture affected by exogenously added different concentrations of JA was similar to regene-ration, initiated in response to different levels of wounding. Platycerium bifurcatum jasmonska kislina ranitev brsti rizoidi aposporni gametofiti intermediati Platycerium bifurcatum jasmonic acid wounding buds rhizoids aposporus gametophytes intermediates true true false [K. Antolin] Slovenski jezik Angleški jezik Diplomsko delo 2011-06-10 08:45:23 2011-06-22 15:21:24 2022-04-13 14:56:07 2023-12-24 03:29:36 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57/59(043.2) 18467592 URN:SI:UM:DK:5QU8XNWN UNI_Antolin_Ksenija_2011.pdf UNI_Antolin_Ksenija_2011.pdf 1 44CE83EDF553088197CACC57C1239D9C a99eb4509985a5781dcfbfd4a3cc7cf5b80d0b362bf5ca7495ebbed5d461da1c 405811d7-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/e59e036c-da76-435d-9034-ee9f905c32fe https://dk.um.si/Dokument.php?lang=slv&id=22681 Fakulteta za naravoslovje in matematiko 0 0 0