20.500.12556/DKUM-19096 KVANTITATIVNO DOLOČANJE REDUKCIJSKIH SLADKORJEV Z DNS REAGENTOM KVANTITATIVE DETERMINATION OF REDUCTIVE SUGARS WITH DNS REAGENT Redukcijski sladkorji so primarni produkt fotosinteze in se uporabljajo za transport molekul za energijo. So vir materialov za tvorbo proteinov, polisaharidov, lipidov in celuloze. Vsebujejo karbonilne skupine, kar pomeni, da delujejo v različnih reakcijah kot redukcijsko sredstvo. Namen te diplomske seminarske naloge je bil poiskati in preizkusiti metodo za kvantitativno določanje redukcijskih sladkorjev v rastlinskem tkivu s pomočjo spektrofotometra SpectroVis. V diplomski seminarski nalogi so podane teoretične osnove o ogljikovih hidratih in spektrofotometriji ter rezultati, analiza in evalvacija preizkusa izbrane metode, ki je najprimernejša za izvajanje na srednjih šolah in fakultetah zaradi izjemne natančnosti, preprostosti in dostopnosti vseh potrebnih materialov in pripomočkov. Reductive sugars are the primary product of photosynthesis and are used to transport molecules for energy. They are a source of materials to form proteins, polysaccharides lipids and cellulose. They contain carbon groups, which means that they work in different reactions as a reductive medium. The purpose of this dissertation is to show and test the method for kvantitative determination of reductive sugars in vegetation tissue with the help of a spectrophotometer SpectroVis. In this graduation seminar thesis we give the theoretical background of carbohydrates and spectroscopy, with the results, analysis and the evaluation of the test of the chosen method. This method is suitable for secondary schools and universities because of its precision, simplicity and accessibility of all necessary materials and equipment. ogljikovi hidrati redukcijski sladkorji spektrofotometrija DNS reagent carbohydrates reductive sugars spectroscopy DNS reagent true true false [S. Zvonar] Slovenski jezik Angleški jezik Diplomsko delo 2011-06-20 13:23:57 2011-06-28 15:17:31 2022-04-13 15:05:15 2023-12-24 03:34:50 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57/59(043.2) 18482184 URN:SI:UM:DK:VBTM0E51 UNI_Zvonar_Sandra_2011.pdf UNI_Zvonar_Sandra_2011.pdf 1 47157CDC0964A4EFFE5D9FCAC9CC5972 20685678a23727f786f84a982a77952a6d6d8c9e626c50289fbb934446cd0435 4a41030f-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/1743af87-fa5b-4bcd-89c7-2a28c7909fcb https://dk.um.si/Dokument.php?lang=slv&id=22929 Fakulteta za naravoslovje in matematiko 0 0 0