20.500.12556/DKUM-20089 Določanje genetske sorodnosti med divjimi malinami (Rubus idaeus L.) in nekaterimi znanimi sortami malin z uporabo mikrosatelitskih markerjev Determination of genetic relationships among wild and some known cultivars of raspberries (Rubus idaeus L.) with microsatellites markers Divje maline so zelo pogosto prisotne trajnice slovenske pokrajine, ki tvorijo naravno populacijo. Zmožnost razlikovanja med posameznimi kultivarji malin, tako gojenimi kot divjimi, je pomembna tako iz znanstvenih kot ekonomskih razlogov. V genetsko analizo smo vključili 6 znanih sort in 19 divjih, ki smo jih nabrali v genski banki, na več območjih Pohorja in v Zasavju. Namen naloge je bil ugotoviti, kakšna je genetska sorodnost znotraj populacij malin, genetska čistost populacije in število močno odstopajočih genotipov znotraj populacije, ki so bili prenešeni iz neke druge populacije. Na dvanajstih mikrosatelitskih lokusih RhM001, RhM003, Rhm011, Rim015, RiM017, RiM019, RhM021, RhM023, RhM043, Rub228, Rub262 in Rub277 smo pri 25-ih preučevanih genotipih divjih in znanih sort malin skupaj namnožili 103 polimorfne alele, v povprečju 8,583 na lokus. Genetske razdalje med proučevanimi genotipi smo izračunali z Diceovim koeficientom in izdelali dendrogram. Wild raspberries are frequently present perennials in the Slovenian landscape and are usually forming vigorous natural populations. The assessment of genetic relationships among cultivated as well as wildly grown genotypes of raspberries (Rubus idaeus L.) is important for scientific and also economic reasons. In our genetic analysis, we included 6 cultivated and 19 wild genotypes which were collected in three different locations in Slovenia. The purpose of the investigation was to analyse genetic similarities and differences of the studied plant materials. We were also interested in the genetic uniformity within populations and genetically atypical genotypes that originated in different populations. Twelve microsatellite loci (RhM001, RhM003, Rhm011, Rim015, RiM017, RiM019, RhM021, RhM023, RhM043, Rub228, Rub262 and Rub277) were taken into account, 25 genotypes of wild and cultivated raspberries were examined and 103 polymorphic alleles were exhibited, which is, in average 8.538 alleles per locus. Genetic distances between studied genotypes were calculated using the Dice coefficient, and a dendrogram was produced. Ključne besede: divje maline Rubus idaeus L. populacija molekulski markerji mikrosateliti genetska sorodnost wild raspberries Rubus idaeus L. population molecular markers microsatellites genetic relationships true true false Slovenski jezik Angleški jezik Magistrsko delo 2011-09-08 10:22:57 2011-09-19 08:26:27 2022-04-14 07:37:19 2023-12-26 03:33:24 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:WQDYRSUW MAG_Weber_Nika_2011.pdf MAG_Weber_Nika_2011.pdf 1 AC0F26FB3035663740A9105C45F1387B e6cd208e87f2c3078ab05142d2b4edc015a8cf0f8e18f6c06d153df63bd63e24 889e2c46-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/51cb092d-cc5d-46d4-ae7b-34b43a28e9f8 https://dk.um.si/Dokument.php?lang=slv&id=24652 Fakulteta za kmetijstvo in biosistemske vede 0 0 0