20.500.12556/DKUM-20413 SINTEZA SAHAROZA PALMITATA V ORGANSKIH TOPILIH SYNTHESIS OF SUCROSE PALMITATE IN ORGANIC SOLVENTS Namen diplomskega dela je bil sintetizirati saharozo palmitat, ki se uporablja v kozmetični, farmacevtski in prehrambeni industriji. Osredotočili smo se na sintezo saharoza palmitata v t-butanolu, vendar smo za primerjavo izvedli tudi sinteze v drugih organskih topilih, kot so etil metil keton, aceton in tert-amil alkohol. Encimsko katalizirano sintezo saharoza palmitata smo izvedli v mešalnem šaržnem reaktorju pri temperaturi 60 °C. Reakcijo smo katalizirali z imobilizirano lipazo B iz Candida antarctica. Produkt reakcije je bil saharoza mono-, di- in tri-palmitat. Reakcije smo izvedli z različnimi množinskimi razmerji substratov (palmitinska kislina : saharoza). Najvišjo presnovo smo dobili v primeru, ko je bilo množinsko razmerje substratov (palmitinska kislina : saharoza) 10 : 90. The purpose of this study was to synthesize sucrose palmitate used in cosmetics, pharmaceutical and food industries. Our focus was mainly on the synthesis of sucrose palmitate in tert-butanol. For comparison the synthesis of sucrose palmitate in other organic solvents like ethyl methyl ketone, acetone and tert-amil alcohol were also carried out. Enzyme catalyzed synthesis of sucrose palmitate was carried out at 60 °C in a batch stirred reactor. Reactions were catalyzed by immobilized lipase B from Candida antarctica. Product was sucrose mono-, di- and three-palmitate. Reactions were carried out with different molar ratio of substrates (palmitic acid : sucrose). The highest conversion was obtained when molar ratio of substrates was (palmitic acid : sucrose) 10 : 90. encimsko katalizirana sinteza lipaza B iz Candida antarctica saharoza palmitat organska topila t-butanol enzymatic synthesis lipase B from Candida antarctica sucrose palmitate organic solvents t-butanol true true false [M. Bahlen] Slovenski jezik Angleški jezik Diplomsko delo 2011-09-19 11:59:05 2011-10-04 14:30:15 2022-04-14 07:54:05 2023-12-27 03:16:03 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.15:547.458.2(043.2) 15835158 URN:SI:UM:DK:AZQJVZX5 UNI_Bahlen_Maja_2011.pdf UNI_Bahlen_Maja_2011.pdf 1 8892B88266587BC4FB6A8C0132642F7F 80f9a5315f2baee8da0fb38df7f76175dda4f8a4b25a481d4c6533768659232b 9a024cd9-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/709f1c73-1e23-44e8-bdfc-8bf55cb3d121 https://dk.um.si/Dokument.php?lang=slv&id=25272 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0