20.500.12556/DKUM-20524 Razmnoževanje čajote (Sechium edule Jacq. Sw.) v in vitro pogojih In vitro propagation of chayote (Sechium edule Jacq. Sw.) V letih 2010 in 2011 smo s tehniko tkivnih kultur v in vitro razmerah razmnoževali dva genotipa čajot (Sechium edule Jacq. Sw.). Posamezne faze mikropropagacije smo najprej izvajali na bodičastih čajotah. Postopke, ki so se izkazali kot najboljši smo na to uporabili pri nebodičastih čajotah. Z metodo sterilizacije DICA smo uspešno sterilizirali 67 % vršičkov rastlin, ki so predhodno rasle v rastlinjaku in 20,2 % vršičkov iz rastlin, ki so rasle na prostem. Sterilizacija z natrijevim in kalcijevim hipokloritom ni bila uspešna. Uspešno sterilizirane vršičke smo prenesli na tri različna gojišča za regeneracijo, ki so se razlikovala po vsebnosti rastnih regulatorjev. Gojišče G0 (z 0,1 mg/l IBA in 0,1 mg/l BAP), se je izkazalo kot slabo, saj so vršički pričeli kalusirati. Gojišči G1 (z 0,01mg/l IAA in 0,1 mg/l kinetina) in G2 (z 0,005 mg/l IAA in 0,05 mg/l kinetina) sta se izkazali kot dobri regeneracijski gojišči. Nekoliko boljše je bilo G1, kjer smo po 2 mesecih dosegli namnožitveni faktor 1,72 v primerjavi z G2, kjer je bil namnožitveni faktor po dveh mesecih 1,66. Tudi koreninjenje je potekalo na treh gojiščih. Na gojišču z dodanim 0,5 mg/l IBA smo pri obeh genotipih dosegli 100 % koreninjenje. Zadnja faza poizkusa je bila aklimatizacija v rastni komori, ki se je izkazala za zelo uspešno. In 2010 and 2011 two genotypes of chayote (Sechium edule Jacq. Sw.) were propagated with the tissue culture technique in in vitro conditions. Each stage of micropropagation was first performed on the barbed chayotes. On unbarbed chayotes, we repeated those procedures which have proved the most success on barbed chayotes. With sterilisation method DICA we successfully sterilized 67% of the explants from plants previously grown in the greenhouse and 20.2% of the explants from plants that were grown outdoors. Sterilisation with sodium and calcium hypochlorite was not successful. Vital sprouts were subcultivated onto three different multiplication media. Medium G0 (with 0.1 mg/l IBA and 0.1 mg/l BAP), medium G1 (with 0.01 IAA and 0.1 mg/ml kinetin) and G2 (with 0.005 IAA and 0.05 mg/ml). On G0 medium the induction of callus were observed. The subcultivation period lasted 2 months and the multiplication factor of 1.72 (medium G1) and 1.62 (medium G2) were obtained. Sufficiently developed plants were transferred to three different rooting media which differed in the presence of auxins (IBA, NAA). In the culture medium with 0.5 mg/l IBA, the percentage of rooted plants was the highest (100 %) in both genotypes. Acclimatisation in the common growth chamber survived 100 % of plants. mikropropagacija in vitro tkivne kulture čajota Sechium edule micropropagation in vitro tissue culture Chayote Sechium edule true true false Slovenski jezik Angleški jezik Diplomsko delo 2011-09-21 13:35:46 2011-10-12 11:45:36 2022-04-14 08:00:14 2023-12-27 03:21:28 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:3EJSYZTT UNI_Verdinek_Erika_2011.pdf UNI_Verdinek_Erika_2011.pdf 1 B33202E656AA73535C115BBF5F882569 7b5b1888592e38eb1752866ecd5b8d76dd41e79daca3473345f2f02337c7dbeb a3bcc9da-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/cd38492e-1948-49d1-a85c-e8c61c87626c https://dk.um.si/Dokument.php?lang=slv&id=25481 Fakulteta za kmetijstvo in biosistemske vede 0 0 0