20.500.12556/DKUM-20623 ELIMINACIJA HMELJEVEGA LATENTNEGA VIROIDA (HLVd) PRI KRIŽANCU HMELJA (Humulus lupulus L.) Z OZNAKO 31/299 ELIMINATION OF HOP LATENT VIROID (HLVd) IN HOP (Humulus lupulus L.) HYBRID 31/299 V diplomskem delu smo na Inštitutu za hmeljarstvo in pivovarstvo Slovenije v Žalcu raziskovali pridobivanje brezviroidnih rastlin iz meristemskega tkiva hmelja, križanca z oznako 31/299. Iz terminalnih in lateralnih brstov je bilo v sterilnih pogojih izoliranih 298 meristemov, ki smo jih položili na MS gojišče z 9µM BAP (6- benzilaminopurin) za iniciacijo brstov (MSB). Od izoliranih 298 meristemov jih je 58 propadlo, 40 jih je zakrnelo, 39 jih je kalusiralo. Od 161 ali 54 % ozelenelih in normalno regeneriranih meristemov jih 65 ali 22 % nismo testirali, 96 ali 33 % pa smo jih analizirali na prisotnost hmeljevega latentnega viroida (HLVd) z metodo RT-PCR in agarozno elektroforezo. Od opravljenih 96 preizkusov prisotnosti HLVd je bilo 9 brezviroidnih križancev hmelja z oznako 31/299. Viroide smo uspešno eliminirali pri 9 oziroma 3 % regenerantov. Eliminacija viroidov je bila uspešnejša pri glavnih poganjkih in manj uspešna pri stranskih poganjkih. The production of viroid-free hop plants of breeding line labeled 31/299 from meristem tissue was studied in the Graduation Thesis. Surveys have been done at the Slovenian Institute of Hop Research and Brewing in Žalec. From the terminal and lateral buds 298 meristems were asseptically isolated and placed on MSB medium for bud initiation with 9µM BAP(6- Benzylaminopurine). From isolated 298 meristems, 58 of them died, 40 of the meristems stunted and 39 callused. From 161 or 54 percentages of green and normal meristems, 65 or 22 percentages were not tested for the presence of HLVd viroid. 96 or 33 percentages of normal meristems were tested for the presence of viroid HLVd by RT-PCR method and agarose electrophoresis. We confirmed the effectiveness of used elimination HLVd method in 9 or 3 percentages of meristems. Elimination of HLVd was more successful in main stems rather than in lateral shoots. žlahtnenje rastlin viroidi HLVd hmeljev latentni viroid hmelj (Humulus lupulus) križanec 31/299 brezviroidne rastline RT-PCR metoda agarozna elektroforeza plant breeding viroids hop latent viroid HLVd hops (Humulus lupulus) hybrid 31/299 viroid free plants RT-PCR agarose electrophoresis true true false [M. Velenšek] Slovenski jezik Angleški jezik Diplomsko delo 2011-09-26 11:47:58 2011-11-18 14:46:06 2022-04-14 08:05:53 2023-12-26 03:34:46 2011 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57/59(043.2) 18771976 URN:SI:UM:DK:VUFARRDN UNI_Velensek_Mihaela_2011.pdf UNI_Velensek_Mihaela_2011.pdf 1 8A1AD6885406890E37AA34289A7A9E20 80bab525e35547d752b5f7e164a5dc3fcc40dbaa731f3e0db36eba7ef38086c5 a98d8c66-7c05-11eb-bb7a-00155d0001ca 20.500.12556/dkum/bae972f8-8f5e-4224-a0e1-3f623e0610cd https://dk.um.si/Dokument.php?lang=slv&id=25659 Fakulteta za naravoslovje in matematiko 0 0 0