20.500.12556/DKUM-22745 SPEKTROFOTOMETRIČNO MERJENJE FLUORESCENCE, FOSFORESCENCE IN SIPANJA SPECTROPHOTOMETRIC MEASUREMENT OF FLUORESCENCE, PHOSPHORESCENCE AND SCATTERING V diplomskem delu obravnavamo energijska stanja v atomih in molekulah, lastnosti fluorescence, fosforescence ter sipanja kot prehode med molekulskimi energijskimi stanji. Opišemo tudi Beer-Lambertov zakon za vse tri pojave. Meritve opravimo s spektrofotometrom MUT Tristan Light in s preoblikovano transmisijsko komoro. Oprema je bila nabavljena v okviru Norveškega finančnega mehanizma. Z meritvami pokažemo Stokesov premik za fluorescenco, spektralni maksimum za fosforescenco in odvisnost intenzitete sipane svetlobe od valovne dolžine. Z meritvami tudi pokažemo, da je prek Beer-Lambertovega zakona mogoče izračunati neznano koncentracijo raztopine tudi iz gostot fluoresciranega oziroma sipanega svetlobnega toka vzorcev. Del diplomskega dela je namenjen zgodovini raziskovanj pojavov in njihovim aplikacijam v moderni znanosti. In the thesis we study and describe the energy levels of atoms and molecules, possible transitions between them, properties of fluorescence, phosphorescence and scattering from the point of view of transitions between molecular energy states. We also present the original form of Beer-Lambert's law as well as its modifications and applications in all three studied phenomena. The measurements were performed with the spectrometer MUT Tristan Light and the transformed transmission chamber. The equipment was acquired within the project financed from the Norway Grants. With the spectrophotometer we can measure the Stoke's fluorescence shift, phosphorescent spectral maximum and the dependence of the intensity of the scattered light with respect to their wavelengths. From the measured spectra of fluoresced or scattered light we were able to determine the concentrations of either fluorescent dyes or small particles in the liquid samples with the use of the modified Beer-Lambert's law. A part of the thesis is dedicated also to the presentation of a historical overview of these phenomena as well as of their applications in modern science. spektrofotometer fluorescenca fosforescenca sipanje absorpcija Beer-Lambertov zakon energijski nivoji spectrophotometer fluorescence phosphorescence scattering absorption light Beer- Lambert's law energy levels true true false [L. Švent] Slovenski jezik Angleški jezik Diplomsko delo 2012-04-19 11:07:07 2012-05-16 12:55:02 2022-04-14 12:04:10 2023-12-30 03:34:31 2012 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 53(043.2) 19132168 URN:SI:UM:DK:8HD8IA7M UNI_Svent_Lucija_2012.pdf UNI_Svent_Lucija_2012.pdf 1 168BD5C6C2B0EE7877E311E44B2D948C 43ddd1cc79878a195a36ce33f777c363fd36c2ac3f91985fe9c35474752d523f 90c459e0-7c06-11eb-bb7a-00155d0001ca 20.500.12556/dkum/baaebeeb-e519-4209-bc5d-0142faee4414 https://dk.um.si/Dokument.php?lang=slv&id=29512 Fakulteta za naravoslovje in matematiko 0 0 0