20.500.12556/DKUM-25758 Ionic liquids as (co)solvents for enzymatic reactions Jonske tečnosti kao ko-solventi za enzimske reakcije Ionic liquids are low melting point salts that represent an excitnq new class of reaction solvents. Many reactions show advantages when carried out in ionic liquids, either with regard to enhanced reaction rates, improved selectivity, or easier reuse of catalysts. To ascertain the influence of ionic liquids on the enzyme activity three different ionic liquids, 1-butyl-3-methylimidazolium chloride ([bmim] [Cl]), 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim] [PF6]) and 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]) were synthesized and investigated as potential media for the hydrolysis of carboxymethyl cellulose, catalyzed by non-immobilized cellulase from Humicola insolens (Celluzyme 0,7T) and for ester synthesis, catalyzed by immobilized lipase from Rhyomucor miehei (Lipozyme RM IM). Enzyme-catalyzed reactions were performed in a batch stirred reactor at atmospheric pressure. Celluzyme 0.7T showed better activity in hydrophobic ionic liquid ([bmim] [PF6]), as compared to hydrophilic ionic liquid ([bmim] [BF4]). In the case of Lipozyme RM IM, the synthetic activity of the enzyme was strongly reduced by incubating the enzyme in ionic liquids. Paper presented at the 1st South-East European congress of chemical engineering, Belgrade, September 25-28, 2005 Jonske tečnosti imaju niske temperature mržnjenja i kao takve predstavljaju novu klasu rastvarača koji se mogu iskoristiti u mnogim reakcijama. Očigledne su prednosti kada se hemijska reakcija izvodi uz prisustvo jonskih tečnosti bilo kod ubrzanja brzine hemijske reakcije, povećane selektivnosti ili jednostavnije ponovne upotrebe katalizatora. U cilju utvrđivanja uticaja jonskih tečnosti na aktivnost enzima, sintetizovane su i ispitane tri različite tečnosti, l-butil-3-meilimidazolijum hlorid ([bmim][CI]) 1-butil-3-metilimidazolijum heksafluorofosfat ([bmim][PF6]) i 1-butil-3-metilimidazolijum tetrafluoroborat ([bmim][BF4]) kao potencijalni rastvarači u reakcijama hidrolize karboksimetil celuloze koja je katalizovana neimobilisanom celulazom iz Humicola insolens (Celluzyme 0,7T) i sintezama estara katalizovanim imobilisanom lipazom iz Rhizomucor miehei (Lipozvme RM IM). Enzimski katalizovane reakcije su izvedene u šaržnom reaktoru pod atmosferskim pritiskom. Pokazalo se da Celluzyme 0,7T ima veću aktivnost u hidrofobnoj jonskoj tečnosti [bmim][PF6]), u odnosu na hidrofilnu ([bmim][BF4]). U slučaju Lipozyme RM IM, aktivnost enzima je značajno smanjena inkubacijom enzima u jonskoj tečnosti. chemical processing ion liquids organic salts biotechnology enzymatic catalysis immobilized lipase stability kemijska procesna tehnika ionske raztopine organske soli biotehnologija encimatska kataliza stabilnost imobilizirane lipaze true false true Angleški jezik Srbski jezik Znanstveno delo 2012-05-31 10:44:07 2012-05-31 10:44:07 2022-04-15 13:13:33 2023-12-31 03:23:00 2006 0 0 str. 181-186 št. 3 Letn. 12 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1451-9372 66.097 10667542 10.2298/CICEQ0603181P 1451-9372 URN:SI:UM:DK:FENCTRNW Chemical_Industry_and_Chemical_Engineering_Quarterly_2006_Paljevac,_Habulin,_Knez_Ionic_liquids_as_(co)solvents_for_enzymatic_reactions.pdf Chemical_Industry_and_Chemical_Engineering_Quarterly_2006_Paljevac,_Habulin,_Knez_Ionic_liquids_as_(co)solvents_for_enzymatic_reactions.pdf 1 4F495A1F799D29FAEAAFD9A0CB74FBFC 74b42169000f4617cb6af1e78a3bf051366287d8aa1882538e648cbbc2287323 136ee650-7c07-11eb-bb7a-00155d0001ca 20.500.12556/dkum/d604a8ac-8c5d-460c-b6df-fe57fa53cff3 https://dk.um.si/Dokument.php?lang=slv&id=114360 http://www.doiserbia.nb.rs/Article.aspx?id=1451-93720603181P 1 https://dk.um.si/Dokument.php?lang=slv&id=114361 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0