20.500.12556/DKUM-26674 Multivariate data analysis of erythrocyte membrane phospholipid fatty acid profiles in the discrimination between normal blood tissue and various disease states The investigation presented here aims to compare the fatty acid composition of red blood cells of patients with different disease states and to test the hypothesis that the changes in fatty acid profiles derived from erythrocyte phospholipids might be relevant to various diseases. The study sample consisted of 342 blood donors, among them 135 with inflammatory bowel disease, 53 with uterine leiomyoma, 14 with verified absence of uterine leiomyoma, 52 with asthma, 18 with colon adenomas, and 70 blood samples without any of mentioned diseases that was used as a control group. After the isolation of erythrocytes from blood samples, total extracted lipids were separated by solid-phase extraction (SPE) into non polar lipids and polar phospholipids. After the saponification of phospholipid fraction, the esterification process followed with boron trifluoride-methanol reagent. The fatty acid methyl ester (FAME) composition of the total red blood cell phospholipid fraction was analyzed by gas chromatography (GC) with flame ionization detector (FID). Additionally two fatty aldehyde dimethyl acetals (hexadecanal and octadecanal dimethyl acetals; 16:0 DMA and 18:0 DMA) derived from erythrocyte membrane plasmalogen phospholipids were also determined. The resulting fatty acid and plasmalogen linked fatty acid composition was evaluated by the principal component analysis (PCA). We demonstrated decreased levels of omega-3 polyunsaturated fatty acids (n-3 PUFAs) in red blood cell membrane of patients with colon adenomas. Also, a large negative correlation was observed among all samples between the quantity of saturated acids and arachidonic (20:4n6) acid as well as saturated acids and adrenic (22:4n6) acid. In PCA score plot a group of female donors is distinguished mainly by the content of linoleic (18:2n6) acid; a small subgroup shows its concentration highly above the average value. At the same time, the same subgroup has both dimethyl acetals below the average concentrations. The study demonstrates feasibility of multivariate data analysis in discrimination of patients with different diseases according to fatty acid profile and suggests considerable differences in membrane fatty acid profiles in patients with various disease states. V prispevku je predstavljena študija primerjave sestave maščobnih kislin iz fosfolipidov človeških membran rdečih krvnih celic bolnikov z izbranimi bolezenskimi stanji z namenom testiranja hipoteze, da je sprememba v sestavi lahko povezana z različnimi boleznimi. V študijo je bilo vključenih 342 posameznikov, od tega 135 bolnikov s kronično vnetno črevesno boleznijo, 53 bolnic z miomi maternice in 14 žensk dokazano brez miomov maternice, nadalje 52 bolnikov z astmo, 18 bolnikov s črevesnimi polipi in 70 zdravih posameznikov (krvodajalcev), ki so služili kot kontrolna skupina. Izolaciji rdečih krvnih celic je sledilo ločevanje celokupnih ekstrahiranih lipidov z ekstrakcijo na trdni fazi (SPE) na nepolarne in polarne lipide. Po umiljenju fosfolipidne frakcije je sledila esterifikacija z bor trifluorid metanolnim reagentom. Sestavo metil estrov maščobnih kislin skupne fosfolipidne frakcije rdečih krvnih celic smo določali s plinsko kromatografijo s plamensko ionizacijskim detektorjem. Dodatno smo iz membran rdečih krvnih celic fosfolipidnih plazmalogenov določili tudi dva dimetil acetala (heksadekanal in oktadekanal dimetil acetal; 16:0 DMA in 18:0 DMA). Z metodo glavnih osi smo karakterizirali vzorce glede na sestavo maščobnih kislin in dimetil acetalov, povezanih s plazmalogeni. Ugotovili smo zelo nizke vsebnosti omega-3 polinenasičenih maščobnih kislin v celičnih membranah rdečih krvnih celic pri pacientih s črevesnimi polipi. Velik negativni korelacijski koeficient je bil ugotovljen med vsebnostjo nasičenih maščobnih kislin in arahidonsko (20:4n6) kislino in med vsebnostjo nasičenih maščobnih kislin in adrensko (22:4n6) kislino. Metoda glavnih osi je pokazala manjšo izstopajočo skupino pacientov ženskega spola z različnim bolezenskim fenotipom, ki se je ločila glede na vsebnost linolne (18:2n6) kisline, ki je bila pri tej podskupini precej nad povprečno vrednostjo vseh 342 vzorcev. Študija je pokazala, da obstajajo razlike v sestavi maščobnih kislin rdečih krvnih celic pri pacientih z različnimi boleznimi. erythrocyte phospholipids cell membrane fatty acid profiles differentiation disease state gas chromatography principal component analysis eritrocitni fosfolipidi celična membrana profili maščobnih kislin diferenciacija stanje bolezni plinska kromatografija analiza glavnih komponent true false true Angleški jezik Slovenski jezik Znanstveno delo 2012-05-31 14:38:23 2012-05-31 14:38:23 2022-04-15 13:32:12 2024-01-03 03:33:47 2010 0 0 str. 571-580 št. 3 Letn. 57 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1318-0207 541 14086149 14417942 1318-0207 URN:SI:UM:DK:L2Z1CY4M Acta_Chimica_Slovenica_2010_Kodba,_Voncina_Multivariate_Data_Analysis_of_Erythrocyte_Membrane_Phospholipid_Fatty_Acid_Profiles_in_the_Di.pdf Acta_Chimica_Slovenica_2010_Kodba,_Voncina_Multivariate_Data_Analysis_of_Erythrocyte_Membrane_Phospholipid_Fatty_Acid_Profiles_in_the_Di.pdf 1 45363F9FDB833E1933D29312B37CDEC9 65335a60386cc0e67721d1339f0fee0963105ad0548d564214fd945d089ec095 8d1b9ba6-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/e39f0539-755d-4682-b8d4-670ec0b8cc7f https://dk.um.si/Dokument.php?lang=slv&id=115871 http://acta-arhiv.chem-soc.si/57/57-3-571.pdf 1 https://dk.um.si/Dokument.php?lang=slv&id=115870 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0