20.500.12556/DKUM-27047 Disodium guanosine 5'-monophosphate self-associates into nanoscale cylinders at pH 8: a combined diffusion NMR spectroscopy and dynamic light scattering study We report a combined NMR and dynamic light scattering (DLS) study on the size of supramolecular structures formed by disodium guanosine 5'-monophosphate, Na2(5'-GMP), at pH 8. In general, two distinct types of aggregate species are present in an aqueous solution of Na2(5'-GMP). One type consists of stacking 5'-GMP monomers, and the other contains stacking G-quartets. Both types of aggregates can be modeled as rodlike cylinders. The cylinder diameter is 10 and 26 A for monomer aggregates and quartet aggregates, respectively. For Na2(5'-GMP) concentrations between 18 and 34 wt %, the cylinders formed by stacking G-quartets have an average length between 8 and 30 nm, corresponding to a stack of ~24-87 G-quartets. These nanoscale aggregates are significantly larger than what had previously been believed for Na2(5'-GMP) self-associationat pH 8. The length of both types of 5'-GMP aggregates was found to increase with Na2(5ć-GMP) concentration but was insensitive to the added NaCl in solution. While the aggregate size for monomer aggregates increases with a decrease in temperature, the size of G-quartet aggregates is essentially independent of temperature. We found that the size of G-quartet aggregates is slightly larger in D2O than in H2O, whereas the size of monomer aggregates remains the same in D2O and in H2O. We observed a linear relationship between the axial ratio of the 5ć-GMP cylinders and the Na2(5'-GMP) concentration for both types of 5ć-GMP aggregates, which suggests a common stacking mechanism for monomers and G-quartets. true false false Angleški jezik Angleški jezik Neznano 2012-06-01 09:42:30 2012-06-01 09:42:31 2022-04-15 03:43:18 2019-07-10 12:11:02 2005 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 539:544 9783574 0002-7863 URN:SI:UM:DK:QRDD4OAF http://dx.doi.org/10.1021/ja042794d 1 https://dk.um.si/Dokument.php?lang=slv&id=36283 Fakulteta za strojništvo 0 0 0