20.500.12556/DKUM-27240-d1276669-88df-d96d-7735-8604d2a7f30a Response of monocyte-derived dendritic cells to rapidly solidified nickel-titanium ribbons with shape memory properties Ni-Ti Shape Memory Alloys (SMAs) have attracted considerable attention as biomaterials for medical devices. However, the biocompatibility of Ni-Ti SMAs is often unsatisfactory due to their poor surface structure. Here we prepared Rapidly Solidified (RS) Ni-Ti SMA ribbons by melt-spinning and their surface was characterised by Augerelectron spectroscopy, X-ray photoelectron spectrometry and scanning electron microscopy. The biocompatibility of the produced ribbons and their immunomodulatory properties were studied on human monocyte-derived dendritic cells (MoDCs). We showed that melt-spinning of Ni-Ti SMAs can form a thin homogenous oxide layer, which improves their corrosion resistance and subsequent toxicity to MoDCs. Ni-Ti RS ribbons stimulated the maturation of MoDCs, as detected by changes in the cells' morphology and increased expression of HLA-DR, CD86, CD40 and CD83 molecules. However, Ni-Ti RS ribbons enhanced the tolerogenic properties of immature MoDCs, which produced higher levels of IL-10 and IL-27, driving the differentiation of IL-10- and TGF-β-producing CD4+T cells. On the other hand, in the presence of lipopolysaccharide, an important pro-inflammatory biomolecule, Ni-Ti RS ribbons enhanced the allostimulatory and Th1 polarising capacity of MoDCs, whereas the production of Th2 and Th17 cytokines was down-regulated. In conclusion, Ni-Ti RS ribbons possess substantial immunomodulatory properties on MoDCs. These findings might be clinically relevant, because implanted Ni-Ti SMA devices can induce both desired and adverse effects on the immune system, depending on the microenvironmental stimuli. nickel-titanium alloy biocompatibility cytokines immunomodulation monocyte-derived dendritic cells nikelj-titanova zlitina biokompatibilnost citokini imunomodulacija monocitne dendritične celice true false true Angleški jezik Slovenski jezik Znanstveno delo 2012-06-01 10:10:44 2012-06-01 10:10:44 2023-08-16 14:40:32 2021-04-11 03:23:13 2012 0 0 str. 58-80 Letn. 23 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1473-2262 669:61 4545306 15705366 10.22203/eCM.v023a05 1473-2262 URN:SI:UM:DK:PQFJBRAW European_Cells_and_Materials_2012_Tomic_et_al._Response_of_monocyte-derived_dendritic_cells_to_rapidly_solidified_nickel-titanium_ribbon.pdf European_Cells_and_Materials_2012_Tomic_et_al._Response_of_monocyte-derived_dendritic_cells_to_rapidly_solidified_nickel-titanium_ribbon.pdf 1 46D6B99513E650ADE5A0C66E33163A24 dd3a0bca40a52d37ba42a4978a1210a56aed6f188bfc480eddc02d17eeddcda1 851259c6-7c0e-11eb-bb7a-00155d0001ca 20.500.12556/dkum/8794315e-04c0-4719-a625-4e4aab07621e https://dk.um.si/Dokument.php?lang=slv&id=115414 http://www.ecmjournal.org/journal/papers/vol023/vol023a05.php 1 https://dk.um.si/Dokument.php?lang=slv&id=36495 Fakulteta za strojništvo 0 0 0