20.500.12556/DKUM-38231 Biološki učinki naravnih mineralnih vod Radenska z ali brez rastlinskih ekstraktov v humanem celičnem modelu Biological effects of natural mineral waters Radenska with or without plant extracts in a human cell model Naravne mineralne vode vsebujejo naravno raztopljene mineralne snovi in imajo zaradi tega dokazane številne prehransko-fiziološke učinke na človekov organizem. Ugotavljali smo specifičen učinek naravnih mineralnih vod Radenska Classic in Naturelle v humanih celičnih modelih v kombinaciji z ali brez ekstraktov vrste Malva sylvestris. Določali smo biološko dostopnost posameznih mineralov, antitumorsko in imunomodulatorno delovanje kombinacij naravnih mineralnih vod in rastlinskih ekstraktov kot tudi njihov vpliv na fiziologijo razvoja intestinalnega epitelija. Dokazali smo, da so minerali vod biološko visoko dosegljivi ter, da ugodno vplivajo na razvoj transepitelijske rezistence intestinalnega epitelija. Hkrati ugotavljamo da naravna mineralni vodi Radenska delujeta antiproliferativno in proapoptotično na celice adenokarcinoma debelega črevesa. Naravni mineralni vodi Radenska Naturelle ter Classic z ali brez ekstraktov gozdnega slezenovca dodatno spodbujata antiinflamatorni Th2 imunski odgovor z zaviranjem proinflamatornega TNF-α ter spodbujanjem antiinflamatornih IL-10 ter TGF-β. Prav tako, zaključujemo, da ekstrakti vrste Malva sylvestris zaradi njihove izjemne biološke aktivnosti še dodatno povečujejo biološki učinek naravnih mineralnih vod Radenska in predstavljajo velik potencial kot funkcionalni prehrambni dodatek. Natural mineral waters contain naturally dissolved minerals and therefore exert beneficial nutritional and physiological effects on the consumer. We have assessed the specific effects of natural mineral waters Radenska Classic and Naturell in combination with or without the extracts of Malva sylvestris in functional human cell models. We assayed the bioavailability of contained minerals, antitumor and immunomodulatory properties of the waters as well as their ability to beneficially influence the integrity of the intestinal epithelia. In our work we have proved that the mineral content of Radenska Classic and Naturelle is highly available and in the same time increases the trans-epithelial potential of intestinal cells. The waters also show an anti-proliferative and proapoptotic effect against CaCo-2 cells. Natural mineral waters Radenska enhance the anti-inflammatory Th2 answer by inhibiting activation of TNF-α and by stimulating anti-inflammatory cytokines IL-10 and TGF-β. We also conclude that the biological activity of mineral waters Radenska can be enhanced by the addition of Malva sylvestris extracts due to their high bioactive potential. mineralna voda Malva sylvestris biološki učinki celični modeli mineral water Malva sylvestris biological activity cell models true true false Slovenski jezik Angleški jezik Magistrsko delo 2012-09-20 12:17:38 2012-10-23 08:58:49 2022-04-25 23:18:37 2021-04-19 03:09:07 2010 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:TSNADI9Q MAG_Trapecar_Martin_2012.pdf MAG_Trapecar_Martin_2012.pdf 1 41D2EC190818CE907F93BB84035DCF9F 18e53482f54224174a9c991f814d8f34451f8a8249c9b43103ce8ba5f0180dea 735962b9-7c08-11eb-bb7a-00155d0001ca 20.500.12556/dkum/12a33ee3-0393-4bad-b633-11d3191d7fd0 https://dk.um.si/Dokument.php?lang=slv&id=50960 Fakulteta za kmetijstvo in biosistemske vede 0 0 0