20.500.12556/DKUM-38561 DOLOČEVANJE MONOSAHARIDOV S KAPILARNO ELEKTROFOREZO DETERMINATION OF MONO-SACCHARIDES USING CAPILLARY ELECTROPHORESIS Namen diplomskega dela je bil s pomočjo kapilarne elektroforeze kvalitativno in kvantitativno ovrednotiti sestavo monosaharidov v vzorcih kefirana. Optimirali smo celoten postopek z uporabo različnih dolžin kapilare ter različnih dodatkov acetonitrila k boratnemu pufru. Vzorce kefirana smo hidrolizirali s H2SO4, derivatizacijo pa smo izvedli z reduktivno aminacijo, ob uporabi natrijevega cianoborohidrida. Rezultati so pokazali, da kefiran vsebuje glukozo in galaktozo. Določili smo, da je skupna vrednost monosaharidov v območju od 29,2 % do 55,8 %. Koncentracija glukoze je bila v območju od 62,8 mg/L do 113,7 mg/L, medtem ko se je koncentracije galaktoze gibala med 55,2 mg/L in 110,1 mg/L. Ugotovili smo, da je najboljša ločljivost komponent potekla ob uporabi kapilare dolžine 37 cm in 30 % dodatkom acetonitrila k boratnemu pufru. The purpose of my diploma thesis was to use capillary electrophoresis in order to evaluate the qualitative and quantitative composition of mono-saccharides in the kefiran samples. We optimized the whole process by using capillary of different lengths and different additions of acetonitrile to the borate buffer. Kefiran samples were hydrolysed by sulphuric acid, and derivatization was performed by reductive amination using sodium cyanoborohydride. The results showed that kefiran contains glucose and galactose. We determined that the total value of monosaccharide was in the range from 29,2 % to 55,8 %. The glucose concentration was in the range from 62,8 mg/L to 113,7 mg/L, while the concentrations of galactose ranged from 55,2 mg/L to 110,1 mg/L. We found that the best resolution of components was established by using a 37 cm long capillary and 30 % acetonitrile addition to the borate buffer. kapilarna elektroforeza elektroosmotski tok monosaharidi kefiran capillary electrophoresis electroosmotic flow mono-saccharides kefiran true true false [N. Virant] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2012-10-02 15:10:43 2012-10-04 15:17:12 2022-05-04 13:23:49 2021-04-19 03:25:37 2012 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.062:577.114.3(043.2) 16710678 URN:SI:UM:DK:2D0J1JZD UNI_Virant_Natalija_2012.pdf UNI_Virant_Natalija_2012.pdf 1 F8C5E7809B5159A9E56C4A558B9F25EB d9a3f6123ebc9d115e033a4fac4d45cd6f9886d82676d60f8ebb5603f0bbb3eb 82e93aa4-7c08-11eb-bb7a-00155d0001ca 20.500.12556/dkum/b6dbcc87-63d9-4a3c-ade1-e8e7d735cd54 https://dk.um.si/Dokument.php?lang=slv&id=51601 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0