20.500.12556/DKUM-38589 UPORABA MEHANSKIH SIL ZA IZOLACIJO ENCIMOV IN PROTEINOV IZ CELIC ČRNE KVASOVKE TRIMMATOSTROMA SALINUM USE OF MECHANICAL FORCES FOR ISOLATION OF ENZYMES AND PROTEINS FROM CELLS OF BLACK YEAST TRIMMATOSTROMA SALINUM Namen diplomske naloge je bil, preučiti vpliv mehanskih sil na izločanje encimov in proteinov iz celic Trimmatostroma salinum pri atmosfereskem tlaku, 35 °C in različnih časih izpostavitve. Rezultati so pokazali, da se je s podaljševanjem časa homogenizacije preživelost celic Trimmatostroma salinum zmanjševala. Določevali smo tudi aktivnost β-glukozidaze, α-amilaze, proteaze, celulaze, ter koncentracijo proteinov pri 595 nm in 280 nm ter merili absorbanco pri 260 nm in 280 nm za določevanje prisotnosti nukleinskih kislin z UV-Vis spektrofotometru. S podaljševanjem časa homogenizacije smo dosegli, da so se iz celic T. salinum izločili intracelularni encimi. The purpose of the diploma thesis was to examine how the secretion of enzymes and proteins are affected by mechanical forces with the extension of homogenization time at 35 °C and atmospheric pressure. The results showed that the survival of Trimmatostroma salinum cells decreased with prolongation of homogenization time at 35 °C. The activity of β-glucosidase, α-amylase, protease, cellulase, concentration of protein at 595 nm and 280 nm, and nucleic acids at 260 nm on UV-Vis spectrophotometer were also measured. With the increase of homogenization time more intracellular enzymes from suspension T. salinum cells were extracted. T. salinum homogeniziranje celulaza proteza β-glukozidaza α-amilaza T. salinum homogenization cellulase protease β-glucosidase α-amylase true true false [K. Grobelnik] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2012-10-03 20:30:11 2012-10-16 14:50:12 2022-05-04 13:25:37 2021-04-19 03:12:40 2012 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.234:579.26(043.2) 16682518 URN:SI:UM:DK:9D4DWH8S UNI_Grobelnik_Kaja_2012.pdf UNI_Grobelnik_Kaja_2012.pdf 1 82E7371E8C88A54FB69CB845F090DB44 c36767e469a596e86ab7c34ddc5b359ea441fd051fea4cbff0329bcfccc7610a 845afea6-7c08-11eb-bb7a-00155d0001ca 20.500.12556/dkum/a0f6351a-38ef-4c84-a76b-990ed9f40cac https://dk.um.si/Dokument.php?lang=slv&id=51645 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0