20.500.12556/DKUM-38692 VPLIV SUPERKRITIČNEGA OGLJIKOVEGA DIOKSIDA NA AKTIVNOST ZAMREŽENIH ENCIMSKIH SKUPKOV (CLEAS) HRENOVE PEROKSIDAZE INFLUENCE OF SUPERCRITICAL CARBON DIOXIDE ON CROSS-LINKED HORSERADISH PEROXIDASE AGGREGATES (CLEAS) ACTIVITY Hrenova peroksidaza je encim, ki ga najdemo v koreninah hrena in so jo preučevali več kot stoletje. S pomočjo njene tridimenzionalne strukture so bile ugotovljene njene uporabne lastnosti. Zlasti je pomembna za farmacevtsko in biotehnološko industrijo, saj je zanimanje za biokatalizatorje v kemijski proizvodnji vedno večje. Industrijsko je pomembna tudi imobilizacija encimov, saj ta zvišuje stabilnost in omogoča ponovno uporabo encima. Ena izmed teh metod je CLEAs, zamreženi encimski skupki, ki predstavlja imobilizacijo brez nosilca. Gre za zamreženje encima. Če takšne skupke hrenove peroksidaze izpostavimo različnim pogojem, kot sta tlak in temperatura, v različnih časovnih intervalih, ugotovimo, da s časom aktivnost agregatov upada. Horseradish peroxidase is an enzyme that could be found in horseradish roots. It has been studied for more than a century. The threedimensional structure of peroxidase revealed a lot of advantages. Because of the interests in biocatalyst, peroxidase is very important in pharmaceutical and biotech industry where immobilization of enzymes is also important. Immobilization increases stability of enzymes and allows re-use. One of immobilization methods is CLEA, cross-linked enzyme aggregates. This method presents carrier-free immobilized enzymes where enzymes are cross-linked. If aggregates of HRP are exposed to different conditions such as pressure and temperature, in different intervals, a decrease in activity of aggregates was detected. hrenova peroksidaza zamreženi encimski skupki imobilizacija brez nosilca superkritični ogljikov dioksid horseradish peroxidase cross-linked enzyme aggregates carrier-free immobilized enzymes supercritical carbon dioxide true true false [K. Kajzer] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2012-10-10 09:41:44 2012-10-16 15:09:37 2022-05-04 13:33:36 2021-04-19 03:30:06 2012 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.473:577.15-987(043.2) 16704534 URN:SI:UM:DK:B7SP26RX UNI_Kajzer_Kaja_2012.pdf UNI_Kajzer_Kaja_2012.pdf 1 635C9163B84693FFD538ADD08189DC34 424ab43649cde2d24a518fe466f3dcf9b0a74365a0a39b4d2b3c65d039b5783b 89dfa447-7c08-11eb-bb7a-00155d0001ca 20.500.12556/dkum/587f8881-b459-4570-a925-5448fdecc2ff https://dk.um.si/Dokument.php?lang=slv&id=51880 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0