20.500.12556/DKUM-39080 TEKOČINSKA KROMATOGRAFIJA Z MASNO DETEKCIJO ZA DOLOČANJE GLUKOZINOLATOV DETERMINATION OF GLUCOSINOLATES USING LIQUID CHROMATOGRAPHY WITH MASS SELECTIVE DETECTION Namen diplomskega dela je bil določiti prisotnost sekundarnih metabolitov rastlin, iz skupine glukozinolatov, v vzorcih različnih križnic. Raziskave so potekale na tekočinskem kromatografu z masno selektivno detekcijo (LC/MS). Najprej smo iz pripravljenih čistih raztopin desetih standardov: glukonasturtin, glukonapin, glukoiberin, sinigrin, glukobrassicin, sinalbin, glukotropaeolin, epiprogoitrin, progoitrin in glukoraphenin določili pogoje, pri katerih bi lahko najučinkovitejše določali glukozinolate. Za dosego optimalnih pogojev smo spreminjali parametre, kot so: vrsta in sestava mobilne faze, kolona, pretok, napetost kolizijske celice, čas trajanja in območje meritev (m/z). Optimalni pogoji, ki smo jih določili, so: mobilna faza H2O z dodatkom metanojske kisline (HCOOH) in amonijevega formiata (CH5NO2), pretok skozi kolono 0,2 ml/min, napetost kolizijske celice 30 V in čas merjenja 30 minut. Ugotovili smo, da so glukozinolati prisotni v realnih vzorcih pri čemer se sestava spojin med vzorci spreminja, koncentracije glukozinolatov pa so praviloma nizke. The purpose of my diploma thesis was to determine the presence of secondary metabolites of plants, from the group of glucosinolates in a sample of cabbage and sacrum. The measurements were carried out on the liquid chromatography with mass selective detection (LC/MS), which allowed a quantitative and structural analysis. From prepared solutions of ten standards; gluconasturtin, gluconapin, glucoiberin, sinigrin, glucobrassicin, sinalbin, glucotropaeolin, epiprogoitrin, progoitrin and glucoraphenin, we first determined the conditions in which glucosinolates could be determined most effectively. To achieve appropriate conditions we changed the following parameters: mobile phase and its composition, the type of column and the flow through it, the voltage of the collision cell, duration and the area of measurements (m/z). Optimal conditions were determined as follows: H2O mobile phase containing formic acid (HCOOH) and ammonium formiate (CH5NO2), the flow through the column 0.2 ml / min, the collision cell voltage of 30 V and the measurement time of 30 minutes. We found that glucosinolates are present in real samples with the composition of the compounds varies between samples, the concentrations of glucosinolates are generally low. HPLC glukozinolati LC/MS HPLC glucosinolates LC/MS true true false [A. Repnik] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2012-11-26 18:55:27 2012-11-28 12:29:11 2022-05-04 14:02:13 2021-04-21 03:17:23 2012 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.85:543.544(043.2) 16683798 URN:SI:UM:DK:NLZZNMLA UNI_Repnik_Aleksandra_2012.pdf UNI_Repnik_Aleksandra_2012.pdf 1 A8B5CA8D52959D29BC23036B8A7BD64B 507d8ca32a9d44d5a37545de46a4536ca40a3cacb5f9e3d7ede488b5a2da7ed4 9cd4d284-7c08-11eb-bb7a-00155d0001ca 20.500.12556/dkum/237dbcc1-e7de-4994-abc7-f56ee645a487 https://dk.um.si/Dokument.php?lang=slv&id=52575 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0