20.500.12556/DKUM-40460 Vsebnost kortizola v slini konj pri terenskem jahanju kot indikator stresnega stanja The content of cortisol in the saliva of recreational trail riding horses as an indicator of the stress state V okviru raziskave smo spremljali koncentracijo kortizola pri 10-letni kobili toplokrvne pasme. Glavni namen raziskave je bil oceniti intenzivnost stresa med rekreativnim terenskim jahanjem. Vzorčili smo zjutraj in zvečer ob isti uri v štirih zaporednih fazah aktivnosti. V prvi fazi so bili vzorci odvzeti, ko je bil konj v svojem bivalnem boksu. Pri drugi fazi smo vzorce jemali med rekreativnim terenskim jahanjem. V tretji fazi so bili vzorci odvzeti 10 minut po končani aktivnosti. Pri četrti fazi pa so bili vzorci odvzeti eno uro po končani aktivnosti. Koncentracije kortizola v slini smo določili s komercialnim kitom (Cortisol ELISA 2009). Povprečne jutranje koncentracije kortizola v mirovanju so bile 0,1358 µg/dL, povprečne večerne 0,0986 µg/dL. Med terenskim jahanjem so bile povprečne jutranje koncentracije kortizola 0,2792 µg/dL ter večerne 0,2733 µg/dL. Najvišje koncentracije kortizola so bile izmerjene 10 minut po končani aktivnosti, kjer so bile povprečne jutranje koncentracije 0,3592 µg/dL, povprečne večerne pa 0,2343 µg/dL. Eno uro po končani aktivnosti so bile povprečne jutranje koncentracije 0,1988 µg/dL, povprečne večerne pa 0,1667 µg/dL. Rezultati so pokazali, da ima dnevno nočni ritem velik vpliv na koncentracijo kortizola v slini, vendar pa sta v našem primeru intenziteta in trajanje aktivnosti dala enak rezultat ne glede na čas vzorčenja. Na osnovi rezultatov sklepamo, da aktivnost živali ni povzročila bistvenega povečanja stresa. In the present study the cortisol level was monitored in a 10-year-old mare of warm-blooded breed. The main purpose of the study was to assess the intensity of stress during recreational trail riding. Samples were taken in the morning and evening at the same time in four successive phases of activities. In the first phase, samples were taken when the horse was in his box stall. In the second phase, the samples were taken during the recreational trail riding. In the third stage, samples were taken 10 minutes after the end of the activity. In the fourth phase, the samples were taken one hour after the end of the activity. Cortisol concentration in saliva was determined using a commercial kit (Cortisol ELISA 2009). Average morning concentration of cortisol during inactivity was 0,1358 µg/dL and average evening concentration was 0,0986 µg/dL. Average morning concentration during trail riding was 0,2792 µg/dL, whereas in the evening the average concentration was 0,2733 µg/dL. Highest concentrations of cortisol were measured 10 minutes after the activity since the average morning concentration measured was 0,3592 µg/dL and average evening concentration 0,2343 µg/dL. An hour after the activity average morning concentration was 0,1988 µg/dL and evening concentration was 0,1667 µg/dL. The results indicated that the day night rhythm has a large effect on the concentration of cortisol in the saliva. However, the intensity and duration of the activity gave the same result irrespective of the time of sampling. Based on our results we can conclude that the activity of the animal did not cause any significant increase in stress. konj rekreativno terensko jahanje stres slina kortizol horse recreational trail riding stress saliva cortisol true true false Slovenski jezik Angleški jezik Diplomsko delo 2013-05-21 15:40:17 2013-06-05 08:57:51 2022-05-12 23:41:30 2021-04-23 03:33:27 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 URN:SI:UM:DK:0TKQMYHU UNI_Lapornik_Andreja_2013.pdf UNI_Lapornik_Andreja_2013.pdf 1 F33AA38ED42F3EA62336460D31FB49D5 b214a62bf0c82a4b17e44b9c9dbfaecd4e80338cf81cd4b4eabd79a92bbec3e3 f41e0a22-7c09-11eb-bb7a-00155d0001ca 20.500.12556/dkum/4a808baf-fbb4-43d3-b980-2a4bb3988acd https://dk.um.si/Dokument.php?lang=slv&id=55372 Fakulteta za kmetijstvo in biosistemske vede 0 0 0