20.500.12556/DKUM-40472 VALIDACIJA METODE ZA DOLOČANJE AFLATOKSINOV V OREŠČKIH S TEKOČINSKO KROMATOGRAFIJO Z UPORABO IMUNOAFINITETNIH KOLON METHOD VALIDATION FOR AFLATOXINS DETERMINATION IN NUTS WITH LIQUID CHROMATOGRAPHY USING IMMUNOAFFINITY COLUMNS Za določanje aflatoksinov G1, G2, B1, B2 v oreščkih smo validirali metodo določanja s tekočinsko kromatografijo z uporabo imunoafinitetnih kolon. Rezultate analiziranih vzorcev smo opredelili s pomočjo umeritvene krivulje v koncentracijah od 1 μg/kg do 10 μg/kg. Dovoljena vsebnost vsote aflatoksinov G1, G2, B1, B2 v oreščkih je 4,0 μg/kg. Rezultate smo ovrednotili glede na predpisane zahteve analizne metode (koncentracijsko območje, meja določanja, meja zaznavnosti, ponovljivost, obnovljivost in pravilnost). Validirana metoda za določanje aflatoksinov G1, G2, B1, B2 v oreščkih s tekočinsko kromatografijo z uporabo imunoafinitetnih kolon je primerna in uporabna, kar smo potrdili z uspešnim sodelovanjem v medlaboratorijskim testom. For the determination of aflatoxin G1, G2, B1, B2 in the nuts using liquid chromatography with immunoaffinity columns method validation was performed. The results of the samples were determined using the calibration curve at concentration range from 1 μg/kg to 10 μg/kg. Permissible content of the sum of aflatoxins G1, G2, B1, B2 in nuts is 4.0 μg/kg. The results were evaluated according to the requirements of the analytical method (concentration range, limit of quantification, limit of detection, repeatability, reproducibility and accuracy). With the results of interlaboratory comparison it is confirmed that the proposed method is appropriate for the determination of aflatoxins G1, G2, B1, B2 in the nuts. aflatoksini oreščki validacija tekočinska kromatografija aflatoxins nuts validation liquid chromatography true true false [B. Bojanc] Slovenski jezik Angleški jezik Diplomsko delo 2013-05-22 17:25:51 2013-05-29 09:19:31 2022-05-12 23:42:08 2021-04-23 03:21:07 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.544:631.576.2(043.2) 17473814 URN:SI:UM:DK:CRSGPMRF UNI_Bojanc_Branka_2013.pdf UNI_Bojanc_Branka_2013.pdf 1 BA980E8335E5F81561B50EE2B9BB8D28 e151fb277aa03dfd94bb23abfd9511a2b9fba2aeb2d10ce71f1cbee17093b484 f4a0cc30-7c09-11eb-bb7a-00155d0001ca 20.500.12556/dkum/be8f4eff-d76f-4a06-9a2f-7cd51f020168 https://dk.um.si/Dokument.php?lang=slv&id=55387 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0