20.500.12556/DKUM-40482 SEPARACIJA FENOLNIH SPOJIN IZ ZELIŠČ SEPARATION PHENOLIC COMPONENTS FROM HERBS Zelišča, ki uspevajo v naših krajih sem ekstrahirala ter analizirala izkoristek ekstrakcije posameznih delov rastline (steblo, listi, cvet) in vsebnost učinkovin. Analizirala sem ekstrakte klasaste mete, koroške mete, dlakave mete, ožepka, sivke in rimske kamilice. Za pridobivanje ekstraktov sem uporabila dve ekstrakcijski metodi, konvencionalno ekstrakcijo pri kateri sem kot topilo uporabila 96% etanol in sem jo izvajala pri sobni temperaturi in pri 40°C ter superkritično ekstrakcijo s CO2 pri 280 bar in 60°C. Pripravljenim raztopinam ekstraktov sem določila vsebnost skupnih fenolov, skupnih flavonoidov ter antioksidativno aktivnost. Uporabila sem spektrofotometrijo in na podlagi dobljenih absorbanc pri različnih valovnih dolžinah izračunala % antioksidativne aktivnosti in na podlagi umeritvenih krivulj določila koncentracijo skupnih fenolov in flavonoidov. Rezultate sem podala na gram ekstrakta in na gram materiala. Antioksidativno učinkovitost ekstraktov sem določila z uporabo DPPH metode. Rezultati analiz vsebnosti antioksidativnih komponent so pokazali, da je v raztopinah ekstraktov, ki sem jih pridobila s konvencionalno ekstrakcijo, višja vsebnost učinkovin kot pa v ekstraktih dobljenih s superkritično ekstrakcijo s CO2. Vsebnost komponent je odvisna tudi od dela rastline, več učinkovin je v listih in v cvetu posameznih rastlin kot pa v steblu. The herbs native to aur region were extracted and the efficiency of the extraction of individual herb parts (stem, leaf, flower) and the content of active ingredients were analysed. The extracts of spike-like mint, carinthian mint, hairy mint, hyssop, lavender and roman chamomile were analysed. Two extraction methods were used; a conventional extraction with 96% ethanol at room temperature and at 40°C and the supercritical extraction with CO2, whic was performed at 280 bar and 60°C. The content of total phenolic compounds and total flavonoids in extracts and antioxidant activity of the extracts were determined by spectrophotometer. Based on the absorbance measured at different wavelengths the % of antioxidant activity of extracts and the concentration of total phenols and flavonoids were determined. The results were expressed per g of extract and per g of material. The antioxidant activity of the extracts was determined by DPPH method. The results of the analysis of antioxidant components show that the solutions of the extracts, gained with the conventional method, have a higher content of active ingredients in comparison to supercritical extraction. The results also vary depending on the part of the plant more active ingredients were extracted from the flowers than from the stems of the plants. Meta sivka ožepek rimska kamilica superkritična ekstrakcija s CO2 konvencionalna ekstrakcija antioksidativna aktivnost skupni fenoli skupni flavonoidi. Mint lavender hyssop roman chamomile supercritical extraction with CO2 conventional extraction antioxidant activity total phenolics total flavonoids. true true false [S. Petek] Slovenski jezik Angleški jezik Diplomsko delo 2013-05-23 13:29:15 2013-05-28 13:12:13 2022-05-12 23:42:32 2021-04-23 03:27:32 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 665.53:547.913(043.2) 17475094 URN:SI:UM:DK:ESCZESJY VS_Petek_Suzana_2013.pdf VS_Petek_Suzana_2013.pdf 1 42C1E786EB86FD6F5A5537E7EC2622C7 f157b55f71d44d3432c6c2877d165a91c3520fb4a4524433d9fa3a1f73bb578d f5a965f4-7c09-11eb-bb7a-00155d0001ca 20.500.12556/dkum/1fb8feba-e21f-4c14-8a05-ecc8733830b5 https://dk.um.si/Dokument.php?lang=slv&id=55411 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0