20.500.12556/DKUM-40724 SPEKTROFOTOMETRIČNO DOLOČANJE FLAVONOIDOV V OGNJIČU (CALENDULA OFFICINALIS) SPECTROPHOTOMETRIC DETERMINATION OF FLAVONOIDS IN MARIGOLD (CALENDULA OFFICINALIS) Calendula Officinalis vsebuje vrsto zdravilnih komponent, kot so čreslovine, grenčine, steroli, smole, terpeni in flavonoidi. Slednji so najpomembnejši, saj imajo močno antioksidativno delovanje, ki je zelo pomembno za zdravje. Vsi skupaj pa dajejo zelišču prav posebno zdravilno moč, ki pospešuje celjenje ran, čisti kri, ureja cirkulacijo, deluje protivnetno in antikarcenogeno. Namen diplomskega dela je bil poiskati, optimirati in delno validirati metodo za določevanje flavonoidov v ognjiču, ter primerjati vsebnost celokupnih flavonoidov v vzorcih, ki smo jih nabirali ob različnih luninih menah. Z omejeno količino vzorcev, ki smo jih vzorčili ob različnih luninih menah, smo izbrali spektrofotometrično metodo, metodo za določevanje flavonoidov z aluminijevim kloridom v metanolu. Da bi ugotovili, ali ima luna kakršenkoli vpliv na količino zdravilnih komponent v rastlinah, smo analizirali vzorce ognjiča, nabrane ob vseh štirih luninih menah; ob prvem krajcu, polni luni, zadnjem krajcu in luni v mlaju. Na podlagi vseh dobljenih rezultatov lahko povzamemo, da se je v 77% analiziranih vzorcev povišana vrednost flavonoidov v ognjiču pojavila pri polni luni,ta delež pa znaša pri suhih vzorcih do 3,7 % in pri svežih vzorcih do 16,1 %, v preostalih analiziranih vzorcih, pa se maksimum vsebnosti flavonoidov pojavi pri prvem krajcu. Metoda z aluminijevim kloridom v metanolu se je izkazala za primerno metodo določevanja flavonoidov v ognjiču. Calendula Officinalis contains many healing components, such as tannins, bitter components, sterols, resins and flavonoids. Flavonoids are the most important as they have strong antioxidant effects, which is very important for health. Owing to all these components, this herb has strong healing powers and it stimulates wound healing, cleanses blood, regulates circulation, and has anti-inflammatory and anti-cancer effects. The aim of this undergraduate thesis is to find, optimize and validate a method for defining flavonoids in the calendula plant and to compare the content of all flavonoids in the samples picked at different lunar phases. With a limited quantity of samples, picked at different lunar phases, we have selected a spectrophotometrical method, i.e. a method for determining flavonoids on the basis of aluminium chloride in methanol. To find out whether the moon has any effect upon the quantity of healing components in plants, we analysed calendula samples, picked at all four lunar phases: at first quarter, full moon, last quarter and new moon. On the basis of the acquired results, it has been established that plants picked at full moon contain more flavonoids than if they were picked at new moon; in dry samples, this portion amounts to 3,7 % and in fresh samples to %. The method based on the aluminium choride in methanol has proved to be suitable for determining the content of flavonoids in the calendula plant. Calendula Officinalis flavonoidi antioksidanti spektrofotometrija lunini vplivi. Calendula Officinalis flavonoids antioxidants spectrophotometry lunar effects. true true false [S. Pirš] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2013-06-11 18:48:52 2013-07-01 10:50:10 2022-05-03 03:05:55 2021-04-22 03:19:45 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.2:633.887(043.2) 17476630 URN:SI:UM:DK:OYAVRS2Q VS_Pirs_Sandra_2013.pdf VS_Pirs_Sandra_2013.pdf 1 D40F600A8BFDE1D8F703F92117F96AC4 d445f4e2a40db6ea8ae23a2e8213e48a8d5eee817c9e47038d8c82eecfb370be 06a70a87-7c0a-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=55958 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0