20.500.12556/DKUM-42211 MERITEV PROSTANOIDOV V KRVNI PLAZMI Z METODO GC/MS Measurement of prostanoids in human blood plasma with the use of gas cromatography and mass spectrometry V seminarju obravnavamo biosintezo, kemijsko zgradbo in pomen eikozanoidov v človeškem organizmu. Preučimo metodo priprave vzorca krvne plazme, postopke meritev ter kvalitativne in kvantitativne analize prostanoidov z uporabo plinskega kromatografa in masnega spektrometra. Na podlagi analize masnih spektrov z uporabo internih standardov določimo koncentracijo prisotnih prostanoidov v vzorcu. In the seminar we study biosynthesis, chemical structure and importance of eicosanoids in humans. We describe the method of a sample preparation from blood plasma, the methods of measurement as well as of qualitative and quantitative analysis of prostanoids with the use of gas cromatography and mass spectrometry. From the obtained mass spectra we determine the concentration of the prostanoids in the sample using the internal standard technique. arahidonska kislina eikozanoidi prostanoidi levkotrieni plinska kromatografija masna spektrometrija interni standard arachidonic acid eicosanoids prostanoids leukotrienes gas cromatography mass spectrometry internal standard true true false [N. Lisac] Slovenski jezik Angleški jezik Delo diplomskega seminarja/zaključno seminarsko delo/naloga 2013-09-13 13:01:01 2014-01-31 14:35:18 2022-05-06 03:08:06 2021-04-24 03:32:53 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 53(043.2) 20282888 URN:SI:UM:DK:EYIJFVNV UNI_Lisac_Nejc_2013.pdf UNI_Lisac_Nejc_2013.pdf 1 6F5BE51265F5389CB2C620ECF18D6F7D e1372ab87be50a31da5f2adb6b19263b82c962664984ef18a667c0d0ca847476 558ff6fb-7c0a-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=58835 Fakulteta za naravoslovje in matematiko 0 0 0