20.500.12556/DKUM-43253 PRIMERJAVA NATANČNOSTI DVEH METOD TESTIRANJA ZA UGOTAVLJANJE PRISOTNOSTI VIRUSA HIV ACCURACY COMPARISON OF TWO METHODS TESTING FOR PRESENCE OF THE HIV VIRUS V diplomski nalogi opisujemo dve metodi testiranja, ki jih uporabljamo za ugotavljanje prisotnosti virusa HIV v krvi krvodajalcev. Eksperimentalni del smo opravljali v Univerzitetnem kliničnem centru Maribor v Centru za transfuzijsko medicino. Opravili smo primerjavo med dvema različnima proizvajalcema reagentov za testiranje na HIV Ag/Ab: Siemens Enzygnost HIV Integral II na analizatorju BEP 2000, ki je specializiran za encimsko imunsko metodo (ELISA) in Abbott HIV Ag/Ab Combo na analizatorju Architect, ki je specializiran za kemilumiscenčno imunsko metodo (CMIA). Testirali smo 103 vzorce, od katerih je bil eden začetno reaktiven s Siemens reagenti, z Abbott reagenti so bili vsi vzorci nereaktivni. In this dissertation we are describing two methods of testing which are used to determine the presence of the HIV virus in the blood of the donors. The experimental part was done in UKC Maribor at the center for transfuzion medicine. We did a comparIson between two different manufacturers of reagents for testing HIV Ag/Ab: Siemens Enzygnost HIV Integral II on the analyzer BEP 2000, which is specialized for enzyme immunoassay (ELISA) and Abbott HIV Ag/Ab Combo on the analyzer Architect , which is specialized for chemiluminescence immunoassay (CMIA). We tested 103 samples of which one was initially reactive with Siemens reagents. With Abbott reagents all of the samples were non-reactive. Through testing we discovered that multiple factors affect the results, the main factors being sensitivity and specificity of the tests. HIV testiranje ELISA CMIA HIV testing ELISA CMIA true true false [S. Bjegović] Slovenski jezik Angleški jezik Diplomsko delo 2013-12-13 10:45:19 2014-01-03 14:32:06 2022-05-09 03:06:34 2021-04-28 03:36:26 2013 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 4864831 URN:SI:UM:DK:HHSW9X2E UNI_Bjegovic_Sanja_2013.pdf UNI_Bjegovic_Sanja_2013.pdf 1 569E1A1CEB3E959A1E234A557C80D3F2 33388af9b78f8510e0e603bf6455ac8a7be6ed41be0cac200c11cf5cee28f978 9bf03552-7c0a-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=61236 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0