20.500.12556/DKUM-45171 OPTIMIZACIJA HIDROLIZE RUTINA V SUBKRITIČNI VODI Z METODO ODZIVNIH POVRŠIN OPTIMIZATION OF RUTIN HYDROLYSIS USING RESPONSE SURFACE METHODOLOGY Cilj diplomskega dela je bil raziskati vpliv reakcijskih parametrov (temperature, časa in koncentracije) na hidrolitsko sposobnost subkritične vode na osnovnem primeru diglikozida kvercetina – rutina. S pomočjo načrtovanja eksperimentov ter metodologije odzivnih površin smo skušali poiskati optimalne reakcijske parametre pri katerih dobimo najvišji izkoristek hidrolize rutina v kvercetin. Vsi eksperimenti so bili izvedeni v visoko-temperaturnem, visoko-tlačnem avtoklavu v inertni (dušikovi) atmosferi pri pogojih, ki smo jih v naprej določili z uporabo računalniškega programa Design-Expert 7.0. S pomočjo tekočinske kromatografije visoke ločljivosti smo določili koncentracijo kvercetina ter drugih produktov hidrotermične degradacije po obdelavi s subkritično vodo. Z uporabo metodologije odzivnih površin smo dobljene rezultate optimizirali in določili optimalne parametre, ki prispevajo k najvišjemu izkoristku kvercetina. The aim of this graduate thesis was to study the influence of reaction parameters (temperature, time and concentration) on the hydrolytic ability of subcritical water on the basic example of diglicoside quercetin – rutin. Highest yield of quercetin was based on experimental design and response surface methodology optimal reaction parameters at wich the highest yield of quercetin is obtained were determined. All experiments were performed in a high-temperature high-pressure autoclave using an inert (nitrogen) atmosphere at condition, which were predefined using Design-Expert 7.0 software. Yield of quercetin and other products of hydrothermal degradation in samples after treatment with subcritical water were determined using high-performance liquid chromatography. Obtained results were optimized using response surface methodology and the optimal parameters, which contributed to the highest yield of quercetin were determined. Subkritična voda Hidrotermična degradacija Rutin Hidroliza Centralno kompozitni načrt Metoda odzivnih površin. Subcritical water Hydrothermal degradation Rutin Hydrolysis Central composite design Response surface methodology. true true false [A. Palko] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2014-08-14 11:08:18 2014-09-11 17:24:34 2022-05-15 03:06:12 2021-05-02 03:20:56 2014 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.094.941:577.164.3(043.2) 18214166 URN:SI:UM:DK:OQCGEKDI UNI_Palko_Anja_2014.pdf UNI_Palko_Anja_2014.pdf 1 DA8FD0A446E96CC48B7813BF9F3A6D2D 48407e1f448f1d7fce95d24e4f6842d712849b40c5a31e7ec8071e61d1e3fade 075de8d0-7c0b-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=65249 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0