20.500.12556/DKUM-46969 Mikropropagacija plazeče zelene (Apium repens (Jacq.) Lag.) ekotip Kew, Velika Britanija Micropropagation of Apium repens ecotype from Kew, United Kingdom Namen naloge je bil vzpostaviti tkivno kulturo plazeče zelene (Apium repens (Jacq.) Lag.) iz semen, pridobljenih iz botaničnega vrta Kew v Veliki Britaniji, in vpeljati postopek hitrega razmnoževanja z mikropropagacijo. Za naše potrebe smo priredili protokol za mikropropagacijo španskega ekotipa plazeče zelene. Ekotip plazeče zelene iz Velike Britanije smo primerjali z ekotipom rastlin iz Španije. Na osnovno gojišče MS smo dodajali različne kombinacije in koncentracije rastnih regulatorjev BAP, 2iP, IBA in NAA. Rast in razvoj smo spremljali tako, da smo merili naslednje lastnosti: višina rastlin, število brstov, število stolonov, število novonastalih brstov na rastlinah in na stolonih, dolžina in število korenin. Plazeča zelena je najbolje uspevala na kontrolnem osnovnem gojišču MS brez rastnih regulatorjev. Največ brstov se je tvorilo na gojiščih MS s kombinacijo 2µM BAP in 0,5µM IBA ter s kombinacijo 2µM BAP in 0,5µM NAA. Korenine so se tvorile na vseh vcepkih na gojišču brez rastnih regulatorjev in z njimi. Zakoreninjene poganjke smo prestavili v zemljo, kjer se je uspešno aklimatiziralo vsaj 50 % rastlin. The aim of the work was to establish a tissue culture and a micropropagation procedure of the creeping marshwort (Apium repens) obtained from The Royal Botanic Gardens Kew in United Kingdom. The objective of our study was to find out if the ecotype from Great Britain behaves the same as the ecotype from Spain. The tissue culture was established from seeds. We tested the effect of different growth regulators on the plant’s growth and development. Growth regulators that were added to the MS basic medium were: BAP, 2iP, IBA and NAA. We measured the plant’s height, the number of stolons, buds and roots and the length and the dispersion of roots. The majority of buds developed on the MS media by the use of the combination 2 µM BAP and 0.5µMIBA and of the combination 2 µM BAP and 0.5 µM NAA. The most successful plants grew on the MS medium without any added growth regulators. All plants developed roots no matter if there were any growth regulators used or not. Rooted plants were transferred to potting soil in which more than 50% of plants successfully survived acclimatization. Apium repens plazeča zelena tkivna kultura mikropropagacija ekotip Apiaceae kobulnice ex situ ohranjanje redke in ogrožene vrste Apium repens tissue culture micropropagation ecotype Apiaceae ex situ conservation rare species true true false [K. Bašič] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2014-12-03 10:28:54 2014-12-22 16:13:21 2022-05-19 03:06:57 2021-04-22 03:23:45 2014 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 581.16:582.795(41)(043.2) 21019400 URN:SI:UM:DK:KQLKC7EV MAG_Basic_Karmen_2014.pdf MAG_Basic_Karmen_2014.pdf 1 516BDC61B706F47E709611F98B3F522B 2203c5fb599d615fef8afbd9a7aecb07f91ce5a3b4c4b77e864ef9fc789bc57c 76043fcd-7c0b-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=69178 Fakulteta za naravoslovje in matematiko 0 0 0