20.500.12556/DKUM-50227 Influence of process parameters on the extraction of flavanones from mandarin peel Flavanones are an important group of flavonoids that are characteristic for citrus. In the present work isolation of flavanones from mandarin peel was performed by conventional extraction using water, ethanol, acetone and aqueous solutions of acetone and ethanol. The extracts were analysed on the content and composition of flavanones. Furthermore the DPPH radical scavenging activity of extracts was determined. Finally, the influence of extraction parameters (particle size, extraction temperature, extraction time,material to solvent ratio, number of extraction stages and type of solvent) on the yield and the efficiency of extraction were determined by Plackett-Burman experimental design. The results showed that 70% aqueous solution of acetone was the most efficient solvent for isolation of flavanones from mandarin peel. The main flavanones present in the obtained extracts were hesperidin (HES) and narirutin (NRT). The number of extraction stages influenced the yield of extraction, type of solvent influenced the hesperidin extraction efficiency and particle size of material influenced the narirutin extraction efficiency. Flavanoni so pomembna skupina flavonoidov, ki so karakteristični za citruse. V naših raziskavah smo s konvencionalno ekstrakcijo izvedli izolacijo flavanonov iz lupin mandarin, pri čemer smo kot topilo uporabili vodo, aceton, etanol ter vodne raztopine acetona in etanola. Dobljene ekstrakte smo analizirali in določili sestavo in vsebnost flavanonov ter določili njihovo delovanje na stabilni DPPH radikal. V nadaljevanju smo s pomočjo Plackett-Burman-ovega eksperimentalnega načrta določili tudi vpliv ekstrakcijskih parametrov (velikost delcev, temperatura in čas ekstrakcije, razmerje material-topilo, število stopenj ekstrakcije ter vrsta topila) na učinkovitost ekstrakcije. Rezultati so pokazali, da je najbolj učinkovito topilo za izolacijo flavonoidov iz lupin mandarin 70% vodna raztopina acetona. Glavna flavanona, prisotna v ekstraktih, sta bila hesperidin in narirutin. Na izkoristek ekstrakcije vpliva število stopenj ekstrakcije, na učinkovitost ekstrakcije hesperidina vrsta topila, medtem ko ima velikost delcev materiala pomemben vpliv na učinkovitost ekstrakcije narirutina. conventional extraction mandarin peel flavanone hesperidin narirutin Plackett-Bauman experimental design konvencionalna ekstrakcija mandarinina lupina flavanon hesperidin narirutin eksperimentalni načrt Plackett-Bauman true false true Angleški jezik Slovenski jezik Znanstveno delo 2015-07-10 12:37:18 2015-07-10 12:37:18 2022-05-29 03:06:22 2021-05-09 03:25:47 2012 0 0 str. 879-887 št. 4 Letn. 59 2012 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1318-0207 66 14086149 16555542 1318-0207 URN:SI:UM:DK:XQBVPJOZ Acta_Chimica_Slovenica_2012_Makovsek,_Knez,_Skerget_Influence_of_Process_Parameters_on_the_Extraction_of_Flavanones_from_Mandarin_Peel.pdf Acta_Chimica_Slovenica_2012_Makovsek,_Knez,_Skerget_Influence_of_Process_Parameters_on_the_Extraction_of_Flavanones_from_Mandarin_Peel.pdf 1 682187AE87B133F7A907D89BDCB5DE2A e1e66460107333fc629d83a123e73486c8b83559aacfd5d8064fb88fd3c36ae0 87ddcf9f-7c0e-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=115529 http://acta-arhiv.chem-soc.si/59/59-4-879.pdf 1 https://dk.um.si/Dokument.php?lang=slv&id=74785 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0