20.500.12556/DKUM-52243 Functionalization of AFM tips for use in force spectroscopy between polymers and model surfaces Funkcionalizacija AFM-konic za uporabo v spektroskopiji sil med polimeri in modelnimi površinami The following work presents the use of two different methods for the attachment of different functional groups onto the AFM tip surface. Such functionalized tips then allow for further binding of molecules with different origins and natures, thus allowing for use when measuring forces, and the extent of interactions appearing between two model surfaces and in real systems. Force spectroscopy, in combination with chemical force microscopy (CFM), as used in this study, exhibits great potential for chemical sensing in the field of polymer sciences. In modern wound treatment, it is very important to know the type and ranges of interactions between different polymer materials, which are mostly crucial components of the dressings. Precise measurement of these interactions would help to choose those materials that fit together without the use of additional chemical modifications on their surfaces. Such modifications are often the cause of unpredictable complications during the course of wound healing. This same method could also be used for interaction evaluation between chosen polymer materials with biological macromolecules, which appear within the wound during the healing process. Such in vitro testing could be of great help when optimal wound dressing materials need to be chosen in order to alleviate a patient s suffering after application. Scanning electron and atomic force microscopies were used in order to prove the effectiveness and applicability of the used functionalization procedures. V prispevku predstavljamo uporabo dveh različnih metod za pripravo AFM-konic z različnimi funkcionalnimi skupinami. Takšna funkcionalizacija je primerna za nadaljnje pripenjanje različnih molekul, kar je osnova za uporabo pri določanju moči in obsega interakcij med modelnimi površinami in v realnih sistemih. Spektroskopija sil v kombinaciji z mikroskopijo na kemijsko silo, kije bila uporabljena v tem delu, ima velik potencial na področju kemijskega zaznavanja v polimerni znanosti. Pri pripravi večplastnih polimernih materialov, ki se uporabljajo pri moderni obravnavi kroničnih ran, nam natančno določanje interakcij lahko pomaga pri njihovi izbiri. Na podlagi takih meritev bi lahko izbrali materiale, ki med seboj najbolje interagirajo, in ne bi bilo treba vpeljati nobene kemijske modifikacije, ki bi lahko negativno vplivala na potek celjenja ran. Prav tako bi bilo mogoče metodo uporabiti za določanje interakcij izbranih polimernih materialov z biološkimi molekulami v rani, s čimer bi in vitro določili optimalen material za plast obliža, ki je v stiku z rano. V delu smo uspešnost in uporabnost funkcionalizacije preverjali z elektronsko mikroskopijo in mikroskopijo na atomsko silo. atomic force microscopy chemical force microscopy force spectroscopy functionalization of AFM tips mikroskop na atomsko silo mikroskop na kemijsko silo spektroskopija sil funkcionalizacija konic true false true Angleški jezik Slovenski jezik Znanstveno delo 2015-07-10 17:17:47 2015-07-10 17:17:47 2022-05-31 03:09:45 2021-05-10 03:15:16 2011 0 0 str. 205-211 št. 3 Letn. 45 2011 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1580-2949 678:535.33 106193664 858282 1580-2949 URN:SI:UM:DK:TRPSF3TI Materiali_in_Tehnologije_2011_Maver_et_al._Functionalization_of_Afm_Tips_for_Use_in_Force_Spectroscopy_Between_Polymers_and_Model_Surfac.pdf Materiali_in_Tehnologije_2011_Maver_et_al._Functionalization_of_Afm_Tips_for_Use_in_Force_Spectroscopy_Between_Polymers_and_Model_Surfac.pdf 1 6AC55EBA518A4812F8E0FFB7834C00C1 b7afba3b9464bf8e15c50099e593b1b3bf241417a8b9baa1fa269d722f2f961b fde089ae-7c0d-11eb-bb7a-00155d0001ca https://dk.um.si/Dokument.php?lang=slv&id=109869 http://mit.imt.si/Revija/izvodi/mit113/maver_T.pdf 1 https://dk.um.si/Dokument.php?lang=slv&id=109870 Fakulteta za strojništvo 0 0 0